固定化纤维素酶对预处理纤维素原料水解效果的影响  

Effect of immobilized cellulase on evaluating hydrolysis efficiency of pretreated cellulose material

作　　者：吕浩[1] 邢楠楠[2] 林增祥[1,3] 杜健[1] 张红漫[4] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]大庆油田有限责任公司供水公司水源开发研究所,大庆163453 [3]中国科学院上海高等技术研究院,上海201203 [4]南京工业大学理学院,南京210009

出　　处：《生物加工过程》2011年第5期38-42,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(20876078);国家高技术研究发展计划(863计划)资助项目(2006AA020103;2006AA02Z240);NSFC-广东联合基金资助项目(U0733001)

摘　　要：以壳聚糖为载体用交联法制备固定化纤维素酶,考察固定化纤维素酶对蒸爆、球磨、超声波、喷淋、高温预处理玉米秸秆纤维素原料的酶解效果。结果表明:物料经蒸爆预处理后酶水解效率最高可以达到95%,球磨预处理水解效率次之,达到60%。用电镜和FT-IR对处理前后秸秆结构进行表征分析,证明预处理对物料的物理结构及化学组成有一定的影响。蒸爆法和球磨法可以使物料致密的天然结构彻底破坏,从而增加物料的比表面积;蒸爆预处理可以使纤维素内部氢键和官能团改变,使物料更易于酶解。Cellulase was immobilized by chitosan gel beads using the cross-linking embedding method. The immobilized enzyme was used to hydrolyze cellulic material corn stover pretreated by steam explosion, ball milling, ultrasonic, spray, and high-temperature processes. Results showed that the steam ex- plosion pretreatment could obtain the best enzymatic hydrolysis efficiency, glucose yield reached 95% ; the ball mill was second to it, glucose yield reached 60%. Meanwhile, the cellulose structures before and after pretreatment were characterized by electron microscopy and FT-IR. It indicated that the physical structure and chemical composition of pretreated materials changed significantly. The steam explosion method and the ball milling method could make the natural structure of the material completely destroy thus increasing the surface area. The internal hydrogen bonds and functional groups of cellulose had been changed during the course of steam explosion, it caused the material more easily to be hydrolized.

关 键 词：壳聚糖 水解 固定化 预处理 玉米秸秆 

分 类 号：Q55[生物学—生物化学]