PEG-6000胁迫下10个苜蓿品种幼苗期抗旱性比较  

Drought tolerance of alfalfa seedlings of 10 varieties under PEG-6000 stress

作　　者：穆怀彬[1] 伏兵哲[2] 德英[1] 

机构地区：[1]中国农业科学院草原研究所,内蒙古呼和浩特010010 [2]宁夏大学农学院,宁夏银川750021

出　　处：《草业科学》2011年第10期1809-1814,共6页Pratacultural Science

摘　　要：为比较苜蓿(Medicago sativa)幼苗抗旱性,采用5种不同水势(0、-0.3、-0.6、-0.9和-1.2 MPa)的PEG-6000溶液对10个苜蓿品种幼苗进行模拟干旱胁迫,测定了细胞膜透性,丙二醛(MDA)、脯氨酸(Pro)和类胡萝卜素(Car)含量以及超氧化物歧化酶(SOD)活性等生理生化指标。结果表明,随着干旱胁迫的加剧,苜蓿幼苗叶片细胞膜透性、MDA和Car含量总体呈上升趋势;Pro含量和SOD活性总体呈先降后升的趋势;苜蓿幼苗期的耐旱极限在-0.9MPa水势附近;利用隶属函数分析得出10个苜蓿品种的抗旱性强弱为:德国德贝>大富豪>阿尔冈金>West blend>FGZT 106>苜蓿王1#>皇后2000>爱菲尼特>新疆大叶>四季旺。An experiment was conducted to estimate the drought tolerance of alfalfa （Medicago sativa） seedlings of 10 varieties stressed by PEG-6000 with the concentrations of 0 （CK）、--0.3、--0.6、-0.9 and -1.2 MPa by measuring cell membrane permeability, malondialdehyde （MDA）, free proline （Pro） and carotenoid （Car） concentration as well as superoxide dismutase （SOD） activity. This study showed that the cell membrane permeability, MDA and Car concentration in alfalfa leaves increased and the Pro concentration and SOD activity firstly decreased and then increased as drought stress increased. The maximum range of drought resistance of alfalfa seedlings was at --0.9 MPa water potential around. The subordination function analysis showed that the drought tolerance of 10 alfalfa varieties was German Derby〉Mil- lionaire〉Algonquin 〉West blend 〉 FGZT 106 〉 Emperor 1#〉 Golden Empress 〉 Affinity〉 XinJiang Daye〉Siriver.

关 键 词：苜蓿 幼苗期 抗旱性 PEG-6000 

分 类 号：S541.9[农业科学—作物学]