参芎化瘀胶囊对血管性痴呆模型大鼠海马CA1区NF-κB p65表达的影响  

Effects of Shenxiong-Huayu capsule on the expression of NF-κB p65 in CA1 area of hippocampus of vascular dementia rats

作　　者：刘斌[1] 赵彬[2] 张玉芹[1] 陶颖媛[1] 毛文静[1] 马原源[1] 

机构地区：[1]河北联合大学附属医院神经内一科,唐山063000 [2]河北联合大学校医院

出　　处：《国际中医中药杂志》2011年第10期887-889,共3页International Journal of Traditional Chinese Medicine

基　　金：河北省医学科学研究重点课题（项目编号：08410）;河北省中医药管理局基金课题（项目编号：2007010）;唐山市卫生临床技术研究课题（项目编号：10150204A-32）

摘　　要：目的探讨参芎化瘀胶囊对血管性痴呆（vascular dementia，VD）大鼠海马CA1区NF-κB p65表达的影响。方法40只SD大鼠随机分为4组：正常组、假手术组、模型组和参芎化瘀胶囊组。采用反复夹闭双侧颈总动脉、同时腹腔注射硝普钠制备血管性痴呆大鼠模型。应用Morris水迷宫检测VD大鼠学习、记忆能力，免疫组化法检测NF-κB p65表达。结果①与正常组、假手术组比较，模型组海马CA1区NF-κB p65阳性细胞[（26．67±5．71）个／高倍视野]明显增多（P均〈0．01）；②与模型组比较，参芎化瘀胶囊治疗组NF-κB p65阳性细胞[（12．83±6．62）个／高倍视野]减少（P〈0．01）。结论参芎化瘀胶囊可能通过减少VD大鼠脑内NF-κB p65的表达发挥脑保护作用，改善VD大鼠的学习、记忆能力。Objective To investigate the effect of Shenxiong-Huayu capsule on the expression of NF-κB p65 in CA1 area of hippocampus of vascular dementia rats. Methods 40 SD rats were evenly divided into 4 groups： normal control group, sham-operation group, model group and Shenxiong-Huayu capsule intervention group. Established vascular dementia rat models by common carotid artery repeatedly occlusion and sodium nitroprusside intraperitoneal injection. The learning and memory abilities of rats were detected by Morris water maze; the expression of NF-r,_B p65 was detected by immtmohistochemistry. Results （1） The expression of NF-κB p65 in the CA1 area of hippocampus was increased in the model group than in the sham-operation group and normal control group （P〈0.01） . （2） The expression of NF-κB p65 in the CA1 area ofhippocampus was decreased in the Shenxiong-Huayu capsule intervention group than in the model group （P〈0.01） . Conclusion Shenxiong-Huayu capsule can reduce the expression of NF-κB p65 protein in CA1 area of hippocampus and improve the learning and memory abilities in vascular dementia rats.

关 键 词：血管性痴呆 NF-ΚBP65 参芎化瘀胶囊 

分 类 号：R285.5[医药卫生—中药学]