质粒pBR322DNA与内切酶EcoRI相互作用的研究  

Study on Interaction between Plasmid pBR322DNA and Endonuclease EcoRI

作　　者：张志勇[1] 王艳伟[1] 杨光参[1] 

机构地区：[1]温州大学物理与电子信息工程学院,浙江温州325035

出　　处：《温州大学学报（自然科学版）》2011年第5期32-37,共6页Journal of Wenzhou University(Natural Science Edition)

基　　金：国家重点基础研究项目(2007CB935900);国家自然科学基金(10974146;20934004);浙江省自然科学基金(Y6090222)

摘　　要：借助原子力显微镜(AFM)对DNA与内切酶的切割与结合作用进行研究.当缓冲液中有Mg2+存在时,内切酶EcoRI会在单一识别位点处切割pBR322DNA,如果缓冲液中的Mg2+用Ca2+代替,内切酶EcoRI则会与pBR322DNA在单一识别位点处结合,并且切割率与结合率都随着内切酶浓度的增加而增加.The interaction of cleaving and binding between DNA and endonucleases EcoRI was observed by atomic force microscope （AFM）. The findings showed that in the presence of Mg2＋ in buffer, endonucleases EcoRI can cleave pBR322DNA on one specific recognition site; while, if Ca2＋ took the place of Mg2＋ in buffer, endonucleases EcoRI can bind pBR322DNA on the specific recognition site. The cleaving and binding ratio would increase with the increase of concentration of endonucleases.

关 键 词：DNA 内切酶 切割 结合 

分 类 号：Q633[生物学—生物物理学]