驼背鲈血细胞的超微结构  

Ultrastructure of peripheral blood cells of highfin grouper Cromileptes altivelis

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作  者:王静香[1,2] 李加儿[1] 区又君[1] 王永翠[1,2] 

机构地区:[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出  处:《南方水产科学》2011年第5期50-54,共5页South China Fisheries Science

基  金:国家高技术研究发展计划(863计划)项目(2006AA10A414);广东省科技计划项目(C20817);广东省重大科技兴海(渔)项目(A200501A04)

摘  要:利用电镜技术对驼背鲈(Cromileptes altivelis)外周血细胞的超微结构进行了研究。结果表明,电镜下可区分6种类型的细胞:红细胞、粒细胞、单核细胞、淋巴细胞、血栓细胞和浆细胞。红细胞呈圆形或椭圆形,可见线粒体、少数囊泡;根据细胞中的颗粒形态大小和细胞核的形态,可将粒细胞分为4种类型:I型粒细胞、II型粒细胞、III型粒细胞和IV型粒细胞。单核细胞圆形,表面较平整,偶有伪足伸出,空泡多见;淋巴细胞的胞质少,仅在细胞核边缘处形成薄薄的一层,细胞器少;血栓细胞异染色质丰富,沿核膜呈带状分布,胞质中有较多大小不等的空泡和少量的线粒体;浆细胞中粗面内质网成层分布包绕在核周围。此外可见到嗜曙红细胞吞噬红细胞、血栓细胞成群聚集分布的现象。Using electron microscopy, we studied the ultrastructure of peripheral blood cells of Cromileptes altivelis. Six types of cells (erythrocyte, granulocyte, monocyte, lymphocyte, thrombocyte and plasma cell) were detected under the electron microscope. Erythrocyte is round or oval with visible mitoehondria and a few vesicles. According to the size, shape of particles and form of nucleus, granulocytes can be divided into 4 types : I-type granulocyte, II-type granulocyte, IIl-type granulocyte and IV-type granulocyte. Monocyte is round with smooth surface and vacuoles, occasionally holding out pseudopodia. Lymphocyte has a few organelles and little cytoplasm which only forms a thin layer around the nuclcolus. Thromboeyte is rich in heterochromatins which distribute zonally along the nuclear membrane. There are many vacuoles at various sizes and a small amount of mitochondria. Rough endoplasmic reticulum distributes hierarchically around the nucleolus in plasma cell. In addition, erythrocytes could be seen to be phagocytized by eosinophils and thrombocytes gathered in groups.

关 键 词:驼背鲈 血细胞 显微结构 

分 类 号:S917.4[农业科学—水产科学]

 

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