实践八号卫星搭载8个苜蓿品种的生理特性与RAPD分析  

Physiological Characteristic and RAPD Analysis of Alfalfa Carried by SJ-8 Recoverable Satellite

作　　者：韩微波[1] 杜连莹[1,2] 张月学[1] 唐凤兰[1] 刘杰淋[1] 刘凤歧[1] 

机构地区：[1]黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086 [2]哈尔滨师范大学,黑龙江哈尔滨150025

出　　处：《黑龙江农业科学》2011年第10期101-103,共3页Heilongjiang Agricultural Sciences

基　　金："十一五"国家科技支撑计划资助项目(2009BAA24B05);"十二五"国家科技支撑计划资助项目(2011BAD17B04);国家航天育种工程地面育种资助项目(2009HT20118);黑龙江省农业科技创新工程资助项目(2010)

摘　　要：为比较不同基因型苜蓿对空间环境响应的差异,探索空间环境诱变紫花苜蓿的特异性及育种前景,利用实践八号(SJ-8)返回式卫星搭载8个苜蓿品种,以未经搭载的种子作为对照,研究空间环境对苜蓿SP1代生理特性和RAPD标记谱带变化的影响。结果表明:经实践八号卫星搭载后,相比对照,8个苜蓿品种的过氧化氢酶(POD)活性增强,超氧物歧化酶(SOD)活性降低,可溶性蛋白含量升高。RAPD标记分析结果表明:实践八号卫星搭载引起了8个苜蓿品种SP1代DNA序列的变化。苜蓿品种WL232、WL323HQ、BeZa87、Pleven6、龙牧801、龙牧803、肇东和草原1号的多态性频率分别为18.06%、14.47%、7.04%、13.70%、15.00%、14.29%、19.23%和11.39%。肇东苜蓿多态性位点频率最高,为19.23%,BeZa87最低,为7.04%。In order to compare the difference of induced response of different gene-type alfalfa,explore space environment on induction characteristic and breeding prospect,the effects of space environment on physiological characteristics and change of RAPD marker were studied.The eight alfalfa cultivars,WL232,WL323HQ,BeZa87,Pleven6,Longmu 801,Longmu 803,Zhaodong and Caoyuan No.1 were loaded,and seeds not carried by satellite were as control.The results showed that POD and soluble protein were higher than control,SOD was lower than control.36 primers were used in RAPD analysis,the polymorphic loci rate of WL232,WL323HQ,BeZa87,Pleven6,Longmu 801,Longmu 803,Zhaodong and Caoyuan No.1 were 18.06%、14.47%、7.04%、13.70%、15.00%、14.29%、19.23% and 11.39%.The polymorphic loci rate of Zhaodong was the highest and BeZa87 was the lowest among eight cultivars.

关 键 词：实践八号 苜蓿 生理特性 RAPD 

分 类 号：S541.9[农业科学—作物学]