Box-Behnken中心组合设计法优化达卡巴嗪脂质体制备工艺  

Optimization of the dacarbazine liposones preparation with Box-Behnken central composite design

作　　者：程桂茹[1] 王淑娟[1] 邹岩[1] 张龙[1] 

机构地区：[1]长春工业大学化学工程学院,吉林长春130012

出　　处：《长春工业大学学报》2011年第4期348-353,共6页Journal of Changchun University of Technology

摘　　要：优化达卡巴嗪脂质体的制备工艺并对其形态及体外释放进行研究。根据Box-Behnken的中心组合实验设计原理设计实验,以包封率、外观形态、体外释放为指标优化脂质体制备工艺。脂质体包封率为94.20%,比预测高0.02%,脂质体粒径在20~50μm。制得的脂质体的体外释放具有突释和缓释特点。预测工艺条件与实际工艺条件之间具有较好的拟合度,Box-Behnken中心组合设计可以应用于达卡巴嗪脂质体工艺条件的筛选。To optimize the DTIC liposome preparation process and study the shape and the properties,we have the experiments according to Box-Behnken central composite principle,with indexes of the encapsulation efficiency,appearance,in vitro release.The Results show that Liposome encapsulation rate is 94.20% which is 0.02% higher than the predicted and the particle size is in 20~50 μm.We come to a conclusion that there are both the burst release and sustained-release in the Liposome,the predicted process conditions is similar to that of the real.The Box-Behnken central composite design can be applied to DTIC liposomes preparation.

关 键 词：达卡巴嗪 脂质体 包封率 Box-Behnken中心组合设计 

分 类 号：TQ460.6[化学工程—制药化工]