甲基苯丙胺诱导的斑马鱼位置偏爱模型研究  

The study on methamphetamine-induced conditioned place preference model in zebrafish

作　　者：陈毅飞[1] 翁建霖[2] 张文清 陈小辉 莫志贤[1] 

机构地区：[1]南方医科大学中医药学院中药药理教研室,广州510515 [2]香港浸会大学生物学系 [3]基础医学院细胞生物教研室

出　　处：《中华行为医学与脑科学杂志》2011年第9期772-774,共3页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金资助项目（30828033）

摘　　要：目的建立甲基苯丙胺诱导的斑马鱼条件性位置偏爱模型。方法根据药物依赖实验研究中的条件性位置偏爱（CPP）原理，设计斑马鱼位置偏爱箱。根据斑马鱼的天然位置偏爱特性，决定其偏爱侧与非偏爱侧。模型组斑马鱼腹腔注射甲基苯丙胺后，放于非偏爱一侧（伴药箱）；注射生理盐水后，放于偏爱一侧（非伴药箱），共训练5d，观察甲基苯丙胺诱导的斑马鱼位置偏爱效应。结果空白对照组造模前后斑马鱼在伴药箱的活动时间无明显变化[分别为（287．5±80．18）S，（276．3±85．04）s]，差异无显著性（P〉0．05）。模型组经5d训练，斑马鱼在伴药箱的活动时间明显延长[（465．5±113．49）s]，与造模前[（247．9±95．62）s]相比，差异有显著性俨〈0．01）；与空白对照组[（276．3±85．04）s]相比，差异也有显著性沪〈0．01）。结论甲基苯丙胺能诱导斑马鱼形成条件性位置偏爱效应，本实验建立的斑马鱼位置偏爱模型可作为一种新的药物依赖动物模型。Objective To establish a methamphetamine-dependent model in zebrabfish. Methods On the basis of conditioned place preference （CPP） in drug-dependent experiment, place preference box for zebrafishes was designed. According to the natural characteristics of zebrafishes, their preference side and non-preferred side were determined. After intraperitoneal injection of methamphetamine, zebrafishes were placed in non-preferred side（ drug box）. After intraperitoneal injection of normal saline, zebrafishes were placed in preference side （non-drug box）. CPP training was five days and then the methamphetamine-induced place preference in zebrafish were observed. Results After five days training, the staying time of zebrafishes of control group in drug box was not significantly lengthened to compared with before training （（ 287.5 ± 80.18 ） s, （276.3 ± 85.04 ） s）, P 〉 0.05 ）. The staying time of zebrafishes of model group in drug box was markedly extended after training. In comparison with before training or control group, the significant differences were observed（ （465.5 ± 113.49） s, （247.9 ± 95.62） s, （276.3 ± 85.04 ） s, P 〈 0.01 ）. Conclusion Methamphetamine can induce conditioned place preference in zebrafishes. The CPP model in zebrafish established in this study can be used as a new animal model in drug dependence.

关 键 词：斑马鱼 条件性位置偏爱 药物依赖 甲基苯丙胺 

分 类 号：R965.1[医药卫生—药理学]