血吸虫可溶性虫卵抗原诱发脑血管内皮细胞缝隙连接蛋白Cx37mRNA及蛋白的表达  

Expression of gap junctional proteins Cx37 mRNA in cerebral arterial endothelium induced by soluble eggs antigen of Schistosome

作　　者：林雪群[1] 薛国勇[2] 祝高春[2] 唐迅[3] 

机构地区：[1]南昌大学医学院基础医学院脑血管研究所,300006 [2]南昌大学医学院基础医学院解剖学教研室,300006 [3]江西护理职业技术学院外科学教研室

出　　处：《中国地方病学杂志》2011年第5期510-513,共4页Chinese Jouranl of Endemiology

基　　金：基金项目：江西省自然科学基金（2009GZY0134）;江西省教育厅科学技术研究项目（赣教技字2007-80）

摘　　要：目的通过观察血吸虫可溶性虫卵抗原（SEA）孵育培养的脑血管内皮细胞缝隙连接（GJ）蛋白的表达和分布，探讨脑动脉GJ在脑血吸虫病病理发生中的作用。方法使用血吸虫SEA孵育培养幼兔脑基底动脉血管内皮细胞，实验分对照组、SEA1～5组：加入SEA（质量浓度分别为10．0％、5．0％、3．3％、2．5％、2．0％），应用逆转录-聚合酶链式反应（RT—PCR）技术测定兔脑血管内皮细胞GJ蛋白Cx37mRNA的表达：应用Western印迹技术测定兔脑血管内皮细胞GJ蛋白Cx37蛋白的表达。结果对照组和SEA1～5组的GJ蛋白Cx37mRNA水平分别为0．239±0．037、0．260±0．043、0．218±0．310、0．647±0．040、0．419±0．036、0．513±0．038：其中SEA3～5组的GJ蛋白Cx37mRNA水平高于对照组（P均〈0．05）。对照组和SEA1～5组GJ蛋白Cx37蛋白表达分别为0．401±0．045、0．485±0．048、0．749±0．052、1．119±0．063、1．015±0．057、0．605±0．047。其中SEA2～5组Cx37蛋白表达高于对照组（P均〈0．05）。结论血吸虫SEA孵育培养的兔脑血管内皮细胞GJ蛋白Cx37mRNA及其蛋白水平高于对照组，提示GJ蛋白在SEA及其分泌物浸润脑动脉沉积于脑组织。从而在诱发脑血吸虫病的病理发生机制中可能起重要的作用。Objective To explore the effects of gap junctional（GJ）proteins in pathogenesis of cerebral schistosomiasis, through observing the expression of gap junctional proteins Cx37 mRNA in cultured cerebral arterial endothelium incubated with soluble eggs antigen（SEA）. Methods Cerebral artery endothelial cells of rabbits were incubated with SEA, and the experiments were divided into control group and SEA 1 - 5 groups （SEA concentrations were 10.0%,5.0%, 3.3%, 2.5%, 2.0%, respectively）, reverse transcription polymerase chain reaction （RT-PCR） and Western blotting were used to examine the expression of Cx37 mRNA and protein in cerebral artery endothelial cells of rabbits, respectively. Results Cx37 mRNA levels of control and SEA 1 - 5 groups were 0.239± 0.037, 0.260 ±0.043, 0.218 ± 0.310, 0.647 ± 0.040, 0.419 ± 0.036, and 0.513 ± 0.038, respectively; SEA 3 - 5 groups were higher than control group of mRNA levels（all P 〈 0.05）. Cx37 protein levels of control and SEA 1 - 5 group were 0.401 ± 0.045, 0.485 ± 0.048, 0.749 ± 0.052, 1.119 ± 0.063, 1.015 ± 0.057 and 0.605 ± 0.047, respectively, of which SEA 2 - 5 groups were higher than control group（all P 〈 0.05）. Conclusions Expression levels of Cx37 mRNA and protein in cultured cerebral artery endothelial cells incubated with SEA are higher than those of control cerebral artery endothelial cells, which suggests that the gap junction proteins may play an important role in pathogenesis of cerebral schistosomiasis through SEA and its secretion in infiltration of brain tissue and deposition in the cerebral arteries.

关 键 词：脑 血管 缝隙连接蛋白类 连接蛋白37 血吸虫病 

分 类 号：R5[医药卫生—内科学]