Bax和Survivin在白藜芦醇抑制人前列腺癌细胞体外增殖中的调节作用  

Roles of Bax and Survivin in the Proliferation of PC-3 Cells Inhibited by Resveratrol in Vitro

作　　者：彭伟[1] 郑航[1] 王行环[1] 郑新民[1] 吕源[1] 

机构地区：[1]武汉大学中南医院泌尿外科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2011年第5期585-588,F0002,共5页Medical Journal of Wuhan University

基　　金：湖北省自然科学基金项目(编号:2007ABA285)

摘　　要：目的:观察白藜芦醇(Res)对人前列腺癌细胞体外增殖的抑制作用,探讨Survivin和Bax在白藜芦醇抑制前列腺癌细胞体外增殖中的调节作用。方法:采用MTT法、流式细胞仪、免疫组化SP法观察了白藜芦醇对前列腺癌PC-3细胞增殖、细胞周期和蛋白表达的影响。结果:白藜芦醇对前列腺癌PC-3细胞增殖的抑制作用明显,呈浓度和时间依赖性。白藜芦醇使前列腺癌PC-3细胞周期阻滞于G0/G1期,且Bax蛋白表达上调,同时,Survivin蛋白表达下降,而Caspase-3活性升高。结论:白藜芦醇能抑制PC-3细胞的增殖,其机制可能是使前列腺癌PC-3细胞阻滞于G0/G1期,且Bax蛋白表达上升,同时Survivin蛋白表达下降,直接激活Caspase-3而抑制PC-3细胞的增殖。Objective：To study the effect of Resveratrol（Res） on the proliferation of human prostate cancer cells in vitro,and to find the effects of Bax and Survivin on these apoptotic cells.Methods：MTT method,flow cytometry（FCM） and SP immunohistochemistry were used to analyze the proliferation,cell cycle and the expression of correlated proteins of apoptosis.Results：Resveratrol showed a significantly inhibitive effect on PC-3 cells in a dose-and-time dependent manner.PC-3 cells treated with Res were arrested in G0/G1 phase in the cell cycle,and the expression of Survivin was down-regulated while the expression of Bax was up-regulated,and the activity of caspase-3 increased.Conclusion：Resveratrol can inhibit the proliferation of PC-3 cells.The likely mechanism is correlated with cancer cell proliferation inhibited by retarding cell cycle,down-regulating of Survivin expression,up-regulating of Bax expression,and activating caspase-3.

关 键 词：白藜芦醇 前列腺癌 BAX SURVIVIN 

分 类 号：R697.3[医药卫生—泌尿科学]