鱼藤酮致帕金森大鼠脑内5-羟色胺和5-羟色胺转运体的表达改变  

Altered expression of serotonin and serotonin transporter in Parkinsonian rats induced by rotenone

作　　者：李双成[1] 石葛明[1] 康云霄[1] 王伟[1] 冯彩婷[1] 王磊[1] 

机构地区：[1]河北医科大学神经生物学研究室,石家庄050017

出　　处：《解剖学报》2011年第5期605-609,共5页Acta Anatomica Sinica

基　　金：河北省自然科学基金资助项目(C2009001094);河北省卫生厅重点课题指导计划资助项目(20090308);河北医科大学科研骨干人才培养计划资助项目(200713)

摘　　要：目的通过观察鱼藤酮处理大鼠脑内相关脑区5-羟色胺(5-HT)和5-羟色胺转运体(SERT)的表达变化,探讨鱼藤酮对5-HT能神经元的影响。方法选用健康成年雄性Wistar大鼠42只,背部皮下注射鱼藤酮制作帕金森病大鼠动物模型,以免疫组织化学染色、免疫印迹法及流式细胞术显示大鼠脑内相关脑区5-HT和SERT的表达变化。结果 1.5-HT:免疫组织化学染色显示,鱼藤酮组大鼠中脑中缝背核5-HT免疫反应阳性神经元数量明显少于对照组(P<0.01),中缝背核和尾壳核5-HT免疫反应强度明显减弱(P<0.01);流式细胞术(间接免疫荧光法)检测显示,鱼藤酮组大鼠5-HT的表达量比对照组明显降低(P<0.01)。2.SERT:免疫组织化学染色显示,鱼藤酮组大鼠中缝背核和尾壳核SERT免疫反应强度明显减弱(P<0.01);免疫印迹法检测发现,鱼藤酮组大鼠中缝背核及尾壳核的SERT与β-actin吸光度比值与对照组相比明显降低(P<0.05)。结论鱼藤酮对5-HT能神经元具有明显的神经毒性。Objective To study whether the expression of serotonin （5-HT） and serotonin transporter （SERT） in the dorsal raphe nucleus （DRN） and caudate putamen （CPu） are altered in Parkinsonian rats induced by rotenone. Methods Forty-two healthy, adult male Wistar rats were divided into the rotenone-treated （21 rats） and the control （21 rats） groups. Rotenone was injected subcutaneously in the back of the rat to establish a Pakinsonian model. Immunocytochemistry, Western blotting and flow cytometry were used to analyze the expression of 5-HT and SERT in the brains of the rats treated by rotenone. Results 1. Immunocytoehemistry showed that the number of 5-HT immunoreative positive neurons in DRN was less in rotenone treated rats than in the control （P 〈 0.01 ） , the immunoreactive intensity of 5-HT in DRN and CPu was weaker in the rotenone treated rat than in the control （P 〈 0. 01 ） ; Flow cytometry showed that the expression of 5-HT in the rotenonc treated rats decreased significantly compared to the control （P 〈 0. 01 ）. 2. Immunocytochemistry showed the immunoreactive intensity of SERT in CPu was weaker in the rotenone treated rat than that in the control （ P 〈 0.01 ） ; Western blotting showed the ratio of SERT/β-actin in the rotenone treated rat was lower than in the control （P 〈 0.05）. Conclusion Rotenone bears obvious toxicity to serotonergic neurons.

关 键 词：鱼藤酮 5-羟色胺 5-羟色胺转运体 中缝背核 尾壳核 免疫组织化学 免疫印迹法 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]