唐氏综合征侯选区域1基因在喉咽鳞状细胞癌与癌旁组织中的表达及意义  

Study of expression and significance of DSCR1 gene in laryngopharynx cancer and peri-cancerous tissues

作　　者：吕超[1,2] 潘新良[1] 高举[2] 李臻[2] 

机构地区：[1]山东大学齐鲁医院耳鼻咽喉科,济南250012 [2]烟台市烟台山医院耳鼻咽喉科,山东烟台264001

出　　处：《临床耳鼻咽喉头颈外科杂志》2011年第18期848-850,共3页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

摘　　要：目的:通过研究喉咽鳞状细胞癌组织与癌旁组织中唐氏综合征侯选区域1(DSCR1)基因的表达,了解其表达与肿瘤临床特征的关系,探讨DSCR1基因对喉咽鳞状细胞癌的生物学行为的影响。方法:免疫组织化学p-V9000法,使用兔抗DSCR1抗体DCT3,检测DSCR1基因蛋白在喉咽鳞状细胞癌与正常癌旁组织中的表达,并将其与临床资料之间进行统计学分析。结果:肿瘤组织中DSCR1基因表达阳性率94.9%,癌旁组织中表达阳性率35.9%,差异有统计学意义(t=23.69,P<0.01);DSCR1基因的表达在不同的病理分化程度、TNM分期差异有统计学意义(P<0.05);DSCR1基因的表达在不同的肿瘤发病年龄、性别、部位、生长方式、淋巴结转移以及吸烟史组均差异无统计学意义。结论:DSCR1基因在喉咽鳞状细胞癌的发生、发展过程中有重要作用,可以影响肿瘤的生物学行为。Objective： To study the expressions of DSCR1 gene in laryngopharynx cancer and peri-cancerous tissues,to understand the relationship between its expression and tumor clinical features,to discusse the influence of DSCR1 gene on the biological behaviours of laryngopharynx squamous cell carcinoma. Method： Immunohistochemical p-V9000 method,used rabbit DSCR1 antibody DCT3,detected the expressions of DSCR1 gene protein in laryngopharynx cancer and peri-cancerous tissues,between them and clinical data were statistically analyzed. Result： Positive rates of DSCR1 gene expression in the tumor tissues was 94.9%,normal tissues was 35.9%,there was a statistically significant（t=23.69,P0.01）;There was significant difference of DSCR1 gene expressions in different pathological degree and TNM staging（P0.05）.There was no significant difference in different age,gender,site,growth mode,lymph node metastasis and smoking history. Conclusion： DSCR1 gene has an important role in the occurrence and development process of laryngopharynx squamous cell carcinoma,and can influence the biological behavior of tumors.

关 键 词：DSCR1基因 肿瘤 鳞状细胞 免疫组织化学 

分 类 号：R766.5[医药卫生—耳鼻咽喉科]