Piper protrusum(Piperaceae),a new species from southern Thailand based on morphological and molecular evidence  

作　　者：Runglawan SUDMOON Tawatchai TANEE Arunrat CHAVEERACH 

机构地区：[1]Department of Biology,Faculty of Science,Khon Kaen University [2]Faculty of Environment and Resource Studies,Mahasarakham University

出　　处：《Journal of Systematics and Evolution》2011年第5期468-475,共8页植物分类学报（英文版）

基　　金：supported by a grant from the Office of the Higher Education Commission Postdoctoral research fund;Part of the research was supported by Khon Kaen University Research Fund academic year2008

摘　　要：Piper protrusum Chaveer. Tanee,sp.nov.is described and illustrated.It dominantly comprises three branching types with three different types of leaf blades,bases,and apexes.The critical distinguishing character is the protruded receptacle having a bract and nine stamens.Individual plants have been discovered in areas of Southern Thailand since 2004 without reproductive parts.The investigated sites were revisited several times,and an individual with ？owers was finally found in July 2009.Phylogenetic analysis of the new species and five similar species is carried out based on DNA fingerprinting.The genetic distances between the new species and five similar species range from 0.25 to 0.35,supporting new species designation.Molecular data conform to morphological data in validating that it is a new species.Additionally,its DNA barcodes have been provided for further identification in case the morphological data is unclear.The sequence data have been submitted to the GenBank database under accession numbers GU980898（rpoB gene）,GU980899（rpoC1 gene）,and GU980900（psbA-trnH region）.Piper protrusum Chaveer. Tanee,sp.nov.is described and illustrated.It dominantly comprises three branching types with three different types of leaf blades,bases,and apexes.The critical distinguishing character is the protruded receptacle having a bract and nine stamens.Individual plants have been discovered in areas of Southern Thailand since 2004 without reproductive parts.The investigated sites were revisited several times,and an individual with ？owers was finally found in July 2009.Phylogenetic analysis of the new species and five similar species is carried out based on DNA fingerprinting.The genetic distances between the new species and five similar species range from 0.25 to 0.35,supporting new species designation.Molecular data conform to morphological data in validating that it is a new species.Additionally,its DNA barcodes have been provided for further identification in case the morphological data is unclear.The sequence data have been submitted to the GenBank database under accession numbers GU980898（rpoB gene）,GU980899（rpoC1 gene）,and GU980900（psbA-trnH region）.

关 键 词：DNA fingerprint new species PIPERACEAE Piper protrusum Thailand. 

分 类 号：Q949[生物学—植物学]