Cloning of a Vacuolar H^+-pyrophosphatase Gene from the Halophyte Suaeda corniculata whose Heterologous Overexpression Improves Salt,Saline-alkali and Drought Tolerance in Arabidopsis  

作　　者：Liang Liu Ying Wang Nan Wang Yuan-Yuan Dong Xiu-Duo Fan Xiu-Ming Liu Jing Yang Hai-Yan Li 

机构地区：[1]Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development,Jilin Agricultural University,Changchun 130118,China [2]College of Life Sciences,Jilin Agricultural University,Changchun 130118,China

出　　处：《Journal of Integrative Plant Biology》2011年第9期731-742,共12页植物学报（英文版）

基　　金：supported by grants from the Program for New Century Excellent Talents in University(Grant No.NCET-080693);the Special Program for Research of Transgenic Plants (Grant No.2008ZX08010-002);the National Natural Science Foundation of China(Grant Nos.30400300 and 30971804);the Science and Technology Development Project of Jilin Province and Changchun City,China(Grant Nos.20080252 and 2009024);the Scientific Research Foundation for Returned Overseas Chinese Scholars,State Education Ministry,China;the State Key Laboratory of Crop Biology at Shandong Agricultural University,China(Grant No.2010KF02)

摘　　要：Salt,saline-alkali conditions,and drought are major environmental factors limiting plant growth and productivity.The vacuolar H＋-translocating inorganic pyrophosphatase（V-H＋-PPase） is an electrogenic proton pump that translocates protons into vacuoles in plant cells.Expression of V-H＋-PPase increases in plants under a number of abiotic stresses,and is thought to have an important role in adaptation to abiotic stress.In this work,we report the isolation and characterization of the gene,ScVP,encoding a vacuolar inorganic pyrophosphatase（V-H＋-PPase） from the halophyte,Suaeda corniculata.Semiquantitative reverse transcription-polymerase chain reaction analysis showed that ScVP was induced in roots,stems and leaves under treatment with salt,saline-alkali and drought.Compared with wild-type（WT） Arabidopsis,transgenic plants overexpressing ScVP accumulated more Na＋ in leaves and roots,and showed increased tolerance to high salinity,saline-alkali and drought stresses.The germination percentage of transgenic Arabidopsis seeds was higher than that of WT seeds under the abiotic stresses.The root length of transgenic plants under salt stress was longer than that of WT plants.Furthermore,the rate of water loss during drought stress was higher in WT than in transgenic plants.Collectively,these results indicate that ScVP plays an important role in plant tolerance to salt,saline-alkali and drought stress.Salt,saline-alkali conditions,and drought are major environmental factors limiting plant growth and productivity.The vacuolar H＋-translocating inorganic pyrophosphatase（V-H＋-PPase） is an electrogenic proton pump that translocates protons into vacuoles in plant cells.Expression of V-H＋-PPase increases in plants under a number of abiotic stresses,and is thought to have an important role in adaptation to abiotic stress.In this work,we report the isolation and characterization of the gene,ScVP,encoding a vacuolar inorganic pyrophosphatase（V-H＋-PPase） from the halophyte,Suaeda corniculata.Semiquantitative reverse transcription-polymerase chain reaction analysis showed that ScVP was induced in roots,stems and leaves under treatment with salt,saline-alkali and drought.Compared with wild-type（WT） Arabidopsis,transgenic plants overexpressing ScVP accumulated more Na＋ in leaves and roots,and showed increased tolerance to high salinity,saline-alkali and drought stresses.The germination percentage of transgenic Arabidopsis seeds was higher than that of WT seeds under the abiotic stresses.The root length of transgenic plants under salt stress was longer than that of WT plants.Furthermore,the rate of water loss during drought stress was higher in WT than in transgenic plants.Collectively,these results indicate that ScVP plays an important role in plant tolerance to salt,saline-alkali and drought stress.

关 键 词：drought H^＋-pyrophosphatase SALT SALINE-ALKALI Suaeda corniculata. 

分 类 号：Q943.2[生物学—植物学] Q949.745.1