针刀松解法对膝骨关节炎大鼠中枢不同部位亮氨酸-脑啡肽的影响  

Affection of acupotomy lysis on Leu-Enkephalin(L-ENK) content in different parts of centrum of rats with knee osteoarthritis

作　　者：郭长青[1] 嵇波[1] 陈幼楠[1] 钟鼎文[1] 金燕[1] 刘清国[1] 郭孟玮[1] 张义[1] 刘乃刚[1] 陈占禄[1] 

机构地区：[1]北京中医药大学针灸推拿学院,北京100029

出　　处：《中国骨伤》2011年第8期656-658,共3页China Journal of Orthopaedics and Traumatology

基　　金：国家重点基础研究发展计划(973计划)(编号:2006CB504508)~~

摘　　要：目的:通过观察针刀松解法干预后膝骨关节炎(KOA)大鼠中枢不同部位亮氨酸-脑啡肽(L-ENK)的变化并与电针干预相比较,探讨针刀松解法在脊髓及脊髓以上水平的镇痛机制。方法:将60只健康SD大鼠随机分为空白组、模型组、电针组和针刀组。将4%木瓜蛋白酶溶液与0.3mol/L半胱氨酸溶液按1:1混合静置0.5h后分别于造模第1、4、7天注射于后3组大鼠左膝关节腔,每次20μl。造模第36天开始,相应给予针刀松解治疗或电针治疗,治疗3周后取大鼠腰膨大处脊髓及脑组织,分别测定脊髓、延髓、中脑、丘脑、海马的L-ENK含量。结果:造模后,模型组大鼠L-ENK含量在脊髓、中脑、海马较空白组差异均有统计学意义(P<0.01),延髓和丘脑L-ENK含量与空白组差异无统计学意义(P>0.05)。针刀和电针干预后,针刀组海马中L-ENK含量较模型组升高,差异有统计学意义(P<0.05);针刀组中脑L-ENK含量较模型组降低,差异有统计学意义(P<0.05);海马中L-ENK含量针刀组较电针组高,差异有统计学意义(P<0.05);其余部位差异均无统计学意义(P>0.05)。结论:针刀松解法对KOA大鼠中枢各水平的L-ENK有一定调节作用,其镇痛作用的发挥可能与其对中枢L-ENK的调节有关。Objective：To study the analgesia mechanism of needle-knife lysis in spinal cord and other parts of central nervous system by comparing the changes of Leu-Enkephalin（L-ENK） content in different parts of centrium of rats undergone needle-knife lysis and electro-acupuncture respectively.Methods：Sixty healthy SD rats were randomly devided into normal control group,model group,needle-knife lysis（NKL） group and electro-acupuncture（EA） group.4% papain solution mixed with 0.3 mol/L cysteine solutin in the ratio of 1：1,paused for 0.5 h,injected the mixture,20 μl each time,into the left knee joint cavities of rats in model,NKL,EA groups at the 1st,4th,7th day.After 4 weeks in NKL group and EA group were treated with needle-knife lysis and electro-acupuncture,respectively.Three weeks after treatment,samples of spinal cord of the swollen part of rat waists and rat brains were taken from and the content of L-ENK of medulla oblongata,midbrain,pituitary gland,and hippocampus were measured.Results：L-ENK content of model group increased higher than that of normal control group in spinal cord,hippocampus and midbrain（P0.01）;there were no significant difference between normal control group and modle group on L-ENK in medulla oblongata and thalamus（P0.05）.After intervening of NKL or EA,L-ENK content of NKL group increased higher in hippocampus than that of model group and EC group（P0.05）;but L-ENK content of NKL group in midbrain was lower than that of model group（P0.05）.Conclusion：Needle-knife lysis has characteristic of regulation for the L-ENK content in different parts of central nervous system of rats with knee osteoarthritis,and analgesic effect of needle-knife was possibly related with regulation of center L-ENK.

关 键 词：骨关节炎 膝 电针 脑啡呔 亮氨酸 动物实验 

分 类 号：R339.55[医药卫生—人体生理学]