Ancient DNA sequences of rice from the low Yangtze reveal significant genotypic divergence  

作　　者：FAN LongJiang GUI YiJie ZHENG YunFei WANG Yu CAI DaGuang YOU XiuLing 

机构地区：[1]Department ofAgronomy, Zhejiang University, Hangzhou 310058, China [2]Zhejiang Provincial Institute of Archaeology and Cultural Relics, Hangzhou 310014, China [3]Department ofMolekulare Phytopathologie, Christian-Albrechts-Universitiit zu Kiel, D-24118 Kiel, Germany [4]School of Humanities, Zhejiang University, Hangzhou 310028, China

出　　处：《Chinese Science Bulletin》2011年第28期3108-3113,共6页

基　　金：the National Basic Research Program of China (2011CB109306);the National High Technology Research and Development Program of China (2006AA10A102);partially by a pilot program (2007DKA20Z90);a DAAD Fellowship

摘　　要：Rice (Oryza sativa) was first domesticated in the lower and middle Yangtze regions of China, and rice remains have been found in many Chinese archaeological sites. Until now, only phenotypic archeobotanical evidence, such as the spikelet bases of ancient grains, has been used to speculate on the domestication process and domestication rate of rice. In this study, we sequenced 4 genomic segments from rice remains in Tianluoshan, a site of the local Hemudu Neolithic culture in the low Yangtze and two other archaeological sites (~2400 and 1200 BC, respectively). We compared our sequences with those of the current domesticated and wild rice (O. rufipogon) populations. At least two genotypes were found in the remains from each site, suggesting a heterozygotic state of the rice seeds. One ancient genotype was not found in the current domesticated population and might have been lost. The rice remains belonged to the japonica group, and most if not all were japonica-type, suggesting that the remains might be at an early stage of indica-japonica divergence or an indica-japonica mixture. We also identified sequences with significant similarity to those from species of Sapindales, Zygophyllales, and Brassicales, which is consistent with the identification of other plant remains in the Tianluoshan site and the common rice field weeds such as mustards in southern China.Rice （Oryza sativa） was first domesticated in the lower and middle Yangtze regions of China, and rice remains have been found in many Chinese archaeological sites. Until now, only phenotypic archeobotanical evidence, such as the spikelet bases of ancient grains, has been used to speculate on the domestication process and domestication rate of rice. In this study, we sequenced 4 ge- nomic segments from rice remains in Tianluoshan, a site of the local Hemudu Neolithic culture in the low Yangtze and two other archaeological sites （-2400 and 1200 BC, respectively）. We compared our sequences with those of the current domesticated and wild rice （O. rufipogon） populations. At least two genotypes were found in the remains from each site, suggesting a heterozygotic state of the rice seeds. One ancient genotype was not found in the current domesticated population and might have been lost. The rice remains belonged to the japonica group, and most if not all were japonica-type, suggesting that the remains might be at an early stage of indica-japonica divergence or an indica-japonica mixture. We also identified sequences with significant similarity to those from species of Sapindales, Zygophyllales, and Brassicales, which is consistent with the identification of other plant re- mains in the Tianluoshan site and the common rice field weeds such as mustards in southern China.

关 键 词：古DNA序列 长江中游地区 基因型 驯化过程 水稻种子 分歧 大米 中国南方 

分 类 号：S511[农业科学—作物学] Q959.878[生物学—动物学]