复发性自然流产患者外周血自然杀伤细胞免疫表型检测  

Immunophenotypes detection of peripheral nature killer cells in patients with recurrent spontaneous abortion

作　　者：谢文锋[1] 严海燕[1] 曾华[1] 梁海霞[2] 丁睿[1] 谢晓英[1] 丁鹤林[1] 

机构地区：[1]中山大学附属第二医院检验科,广州510120 [2]中山大学附属第二医院生殖中心,广州510120

出　　处：《中华生物医学工程杂志》2011年第4期351-354,共4页Chinese Journal of Biomedical Engineering

摘　　要：目的 检测复发性自然流产（RSA）患者外周血自然杀伤（NK）细胞的免疫表型。方法 应用流式细胞术检测27例RSA妊娠患者、41例RSA非妊娠患者、32例正常妊娠者和25例正常非妊娠者外周血CD56dimCD 16＋、CD56brightCD16＋/-、CD69＋、HLA-DR＋ NK细胞表达并进行比较。结果RSA妊娠组、RSA非妊娠组、正常妊娠组和正常非妊娠组外周血CD56dimCD16＋ NK细胞占总NK细胞的比例分别为（88.69.±5.86）％、（79.25±9.31）％、（79.24±10.09）％、（75.49±11.96）％;CD56brightCD16＋/- NK细胞的比例分别为（8.18±5.54）％、（ 12.20±6.49）％、（ 13.13±8.65）％、（ 11.53±6.23）％；CD69＋ NK细胞的比例分别为（3.42±2.13）％、（2.36±1.72）％、（2.68±1.81）％、（2.08±1.73）％；HLA-DR＋ NK细胞的比例分别为（15.26±9.32）％、（9.96±7.18）％、（ 10.57±8.05）％、（9.64±6.12）％。与RSA非妊娠组、正常妊娠组及正常非妊娠组比较，RSA妊娠组CD56dimCD16＋、CD69＋、HLA-DR＋ NK细胞显著升高（均P＜0.05）。与RSA非妊娠组及正常妊娠组比较，RSA妊娠组CD56brightCD16＋/- NK细胞明显降低（均P＜0.05）。结论 RSA妊娠患者外周血中NK细胞CD56dimCD16＋、CD69＋和HLA-DR＋表达增高，而CD56brightCD16＋/-表达下降，可能在RSA的发生发展中起着一定的作用。Objective To determine the immunophenotypes of peripheral nature killer （NK） cells in patients with recurrent spontaneous abortion （RSA）. Methods Flow cytometry was used to determine the expressions of CD56dimCD16＋, CD56brightD16＋/-, CD69＋ and HLA-DR＋ NK cells in peripheral blood samples from 27 pregnant and 41 non-pregnant RSA patients, 32 normal pregnant and 25 non-pregnant women, and the expressions were compared among 4 groups. Results The percentage of total peripheral NK cells from pregnant and non-pregnant RSA women, normal pregnant and non-pregnant women was （88.69± 5.86）%, （79.25±9.31 ）%, （79.24±10.09）% and （75.49±11.96）%, respectively, for CD56dimCD16＋ NK cells; （8.18 ± 5.54）% , （12.20 ± 6.49）% , （13.13 ± 8.65）% and （11.53 ± 6.23）% , respectively, for CD56brightCD16＋/- NK cells; （3.42±2.13）% , （2.36± 1.72）%, （2.68± 1.81）% and （2.08± 1.73）% ,respectively, for CD69＋ NK cells; （15.26±9.32）%, （9.96±7.18）%, （10.57±8.05）% and （9.64±6.12）%,respectively, for HLA-DR＋ NK cells. Compared with non-pregnant RSA women, normal pregnant or non-pregnant women, pregnant RSA women showed significantly higher percentage of CD56dimCD16% CD69＋ and HLA-DR＋ NK cells （all P〈0.05）. Compared with non-pregnant RSA and normal pregnant women, pregnant RSA women showed significantly lower percentage of CD56brightCD16＋/- NK cells （both P〈0.05）.Conclusions The increased expression of CD56dimCD16＋, CD69＋ and HLA-DR＋ NK cells and decreased expression of CD56brightCD16＋/- NK cells in peripheral blood of the RSA pregnant patients may play a role in the development and progression of RSA.

关 键 词：流产 复发性 流式细胞术 自然杀伤细胞 抗原 CD69 组织相容性抗原 

分 类 号：R714.21[医药卫生—妇产科学]