筛选与拟南芥绒毡层发育过程中重要的转录因子DYT1相互作用的调控蛋白因子(英文)  

Screening for the regulatory protein factors interacting with core transcription factor DYT1 for Arabidopsis tapetum development

作　　者：孙立成[1] 韦嘉励[1] 褚艳霞[1] 周树敏[1] 袁晓君[1] 张卫[1] 

机构地区：[1]上海大学上海市能源作物育种及应用重点实验室,上海200444

出　　处：《上海师范大学学报（自然科学版）》2011年第2期179-185,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：The Science and Technology Commission of Shanghai Municipality(08PJ1405500);The National Natural Science Foundation of China(30870225);The Shanghai Municipality Education Commission 2009 Innovation Project of Science and Technology

摘　　要：DYT1是拟南芥绒毡层发育过程中重要的转录因子.DYT1的缺失会导致绒毡层相关功能的缺陷.然而,DYT1并不是使绒毡层功能化的充分因素,暗示了DYT1蛋白需要和其他转录因子形成复合物才能发挥功能.利用酵母双杂交技术以DYT1蛋白为诱饵在拟南芥cDNA文库中筛选与DYT1相互作用的蛋白.通过筛选从文库中得到20个阳性克隆,通过BLAST序列比对得到8个候选基因序列,从中鉴定出6种候选基因编码可能的转录因子或者其他调控转录的蛋白因子.其中5个编码调控因子的基因都被证实在花药中表达.更进一步,发现另一个对花药发育所必需的转录因子AMS能与DYT1在酵母体内形成转录复合物.表明这2个蛋白可能形成异源复合物在花药中共同调节下游基因的表达.DYT1 is a core transcription factor for Arabidopsis tapetum development.Impairment of DYT1 function causes failure of most tapetum-related functions.However,DYT1 alone is not sufficient for overall tapetum function,suggesting there should be other factors DYT1 works with.Using DYT1 as bait in yeast two-hybrid system,screening for the protein interacting with DYT1 was performed in this report.20 positive colonies were obtained.Blast sequence comparison acquired 8 candidate sequences,from which 6 candidates were exhibited to encode putative transcription factors or other transcription-regulating factors.5 of these regulatory genes were confirmed to be expressed in anther.Furthermore we found another anther indispensable transcription factor AMS could interact with DYT1 in yeast,suggesting that these two transcription factors form heterozygous transcription complex to control the expression of downstream genes in anther development.

关 键 词：拟南芥 绒毡层 DYT1 AMS 酵母双杂 

分 类 号：Q37[生物学—遗传学]