腹水高迁移率族蛋白B1检测及其意义  

Determination of high mobility group protein B1 in ascitic fluids and its clinical significance

作　　者：王婷婷[1] 朱松涛[1] 陈云清[1] 胡志刚[1] 周颖[1] 陈国千[1] 

机构地区：[1]南京医科大学附属无锡人民医院医学检验科,江苏无锡214023

出　　处：《中华医院感染学杂志》2011年第19期3989-3991,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金(81070074/H0116)

摘　　要：目的探讨不同性质腹水中高迁移率族蛋白B1(HMGB1)水平的变化及其临床意义。方法采用ELISA法对74份临床腹水标本(其中漏出液22份、炎症性渗出液23份、肿瘤性渗出液29份)进行HMGB1检测,并与白细胞计数作相关性分析。结果炎症性渗出液和肿瘤性渗出液的HMGB1水平明显高于漏出液,差异有统计学意义(P<0.01),而炎症性渗出液的HMGB1/总蛋白比值高于肿瘤性渗出液(P<0.01);腹水HMGB1对渗出液鉴别灵敏度为90.0%时,其特异性约为80.0%;渗出液中HMGB1水平与白细胞计数间具有正相关性(r=0.522,P<0.01)。结论腹水HMGB1检测对于腹水性质的鉴别具有较好的临床意义。OBJECTIVE To investigate change of HMGB1 level in ascitic fluids and its clinical significance.METHODS HMGB1 in 74 specimens of ascitic fluids（22 transudates,23 infectious exudates and 29 malignant exudates） was determinated by ELISA,and was statistically analysed for its correlativity with white cell count.RESULTS The average level of HMGB1 in infectious exudates and malignant exudates was significantly higher than that in transudates（P0.01）.The rate of HMGB1/total protein in infectious exudates was higher than in malignant exudates（P0.01）.The sensitivity and specificity of HMGB1 level in ascitic fluids were about 90.0% and 80.0% for exudate differentiation,respectively.There was significant positive correlation between HMGB1 level and white cell count in exudates（r=0.522,P0.01）.CONCLUSION Determination of HMGB1 in ascitic fluids has potential clinical significance to the differentiation of ascitic fluid types.

关 键 词：高迁移率族蛋白B1 腹腔积液 ELISA 

分 类 号：R446.62[医药卫生—诊断学]