甘露寡糖对草鱼血清生化特性的影响  

Effects of prebiotic mannanoligosaccharide on biochemical properties of serum from grass carp,Ctenopharyngodon idellus

作　　者：陈希[1] 吴志新[2] 刘佳佳[2] 彭小云[2] 袁娟[2] 朱旭[2] 于艳梅[2] 

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]华中农业大学水产学院,湖北武汉430070

出　　处：《南昌大学学报（理科版）》2011年第4期393-397,402,共6页Journal of Nanchang University(Natural Science)

基　　金：国家科技支撑计划基金资助项目(2007BAD37B02);湖北省"十一五"科技攻关基金资助项目(2007AA203A01);中国水产科学研究院淡水生态与健康养殖重点开放实验室开放课题(2010FE03013)

摘　　要：以体重100 g左右的草鱼（Ctenopharyngodon idellus）为实验对象,在基础饲料（A0组）中分别添加浓度为1.0 g/kg（A1组）、2.0 g/kg（A2组）、3.0 g/kg（A3组）、4.0 g/kg（A4组）的甘露寡糖（Mannoseoligosaccharides,MOS）,分别于0、14、285、6 d取样,测定甘露寡糖对草鱼生化特性的影响。结果表明：饲料中添加2.0~3.0 g/kg的甘露寡糖在实验的第28~56 d,草鱼血清中血糖的含量、总胆固醇含量显著降低,第14 d起甘油三酯的含量均显著低于对照组,56 d时血清总蛋白含量显著高于对照组（P〈0.05）;在14~28 d,添加2.0~4.0 g/kg甘露寡糖,草鱼血清碱性磷酸酶活性显著高于对照组（P〈0.05）。添加量为2.0 g/kg时,草鱼的增重率最大。各试验组血清中谷草转氨酶、谷丙转氨酶活性有所下降,但未见显著性差异（P〉0.05）。In the present study,the effects of prebiotic mannanoligosaccharides（MOS） on the biochemical properties of serum from grass carp,Ctenopharyngodon idellus,were investigated.The experimental fish were divided into four groups,with triplicates per treatment.The control group（A0） wasfed basal diet,three treatments（A1,A2 and A3） fed the diets containing 1,2,3 g/kg MOS,respectively.Samples were collected at different time points,such as at 0 d,14 d,28 d and 56 d.It was found that when feeding with 2.0 g/kg and 3.0 g/kg MOS,the blood glucose content and the levels of total cholesterol（CHO） in serum of grass carp were lower than those fed the basal diet（P0.05） from 28 to 56 d,the levels of serum triglycerides（TG） were also lower than those on the basal diet（P0.05） from 14 to 56 d.However,total protein（TP） contents were higher than those fed the basal diet at 56 d（P0.05）.Feeding with 2.0-4.0 g/kg MOS,alkaline phosphatase（ALP） activity in serum were found increased when from 14 to 28 d（P0.05）.The glutamic-oxaloacetic transaminase（GOT）,glutamic-pyruvic transaminase（GPT） activity did not show significant change（P0.05） from 14 to 56 d.Compared to Ao grap Moreover,2.0 g/kg MOS was the most effective diet to increase the weight of grass carp as fonnd in this study.

关 键 词：甘露寡糖 生化特性 草鱼 

分 类 号：Q958[生物学—动物学]