小鼠B7-H3增强体外脂多糖介导的单核细胞活化  

Murine B7-H3 Enhancing to the Lipopolysaccharide Activated Monocytes

作　　者：孙静[1,2] 严茹红[2,3] 顾文超[2] 张光波[2] 傅丰庆[2] 张学光[2] 

机构地区：[1]苏州卫生职业技术学院,江苏苏州215009 [2]苏州大学医学生物技术研究所,江苏苏州215007 [3]苏州大学附属第二医院,江苏苏州215004

出　　处：《苏州大学学报（医学版）》2011年第4期535-537,585,共4页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(31040022)

摘　　要：目的利用已成功构建的小鼠B7-H3融合蛋白通过体外实验研究B7-H3对单核细胞活化及功能的调节作用。方法采用磁珠法从小鼠脾脏中分离得到CD11b+单核细胞,经脂多糖活化后通过融合蛋白结合实验检测单核细胞表面B7-H3受体的表达;加入不同剂量小鼠B7-H3融合蛋白培养,ELISA法检测单核细胞IL-1β、TNF-α与IL-6的分泌,并通过吞噬实验分析其对单核细胞吞噬功能的影响。结果活化的小鼠单核细胞表面可能存在B7-H3的未知受体;与对照相比,小鼠B7-H3可以增加活化的单核细胞IL-1β、TNF-α与IL-6的分泌(均P<0.01),并增强单核细胞的吞噬能力。结论小鼠B7-H3分子在感染免疫中具有重要作用,可参与疾病的抗感染功能的发挥。Objective To discuss the B7-H3 regulation and function to monocytes using murine B7-H3 fusion protein.Methods CD11b＋ cells were separated from splenocyte suspensions using a mouse CD11b positive selection kit,then the expression of B7-H3 receptor on monocytes was analyzed after the cells was activated by lipopolysaccharide for 24 hours.Cells were incubated with B7-H3-Ig-fusion protein with LPS for 24h and the IL-1β,TNF-α and IL-6 were detected through ELISA.At the same time the changes were measured in phagocytic activity of monocytes with FITC-dextran.Results There might be a potential receptor for B7-H3 on mouse monocyte.Murine B7-H3 could increase the secreting of IL-1β,TNF-α and IL-6 relative to the controls.Additionally the gene could enhance the phagocytosis of monocyte.Conclusion Murine B7-H3 plays an important role in infective immunity to take part in the disease counteracting.

关 键 词：小鼠B7-H3 单核细胞 感染免疫 脂多糖 

分 类 号：R392.12[医药卫生—免疫学]