三种鉴别诊断PRRSV美洲型经典毒株和变异株的方法比较  

A COMPARATIVE STUDY OF THREE DIAGNOSTIC METHODS FOR DIFFERENTIAL DETECTION OF CLASSICAL PRRSV AND PRRSV VARIANT

作　　者：陈仕龙[1,2] 陈少莺[1,2] 江斌[1] 林锋强[1,2] 朱小丽[1,2] 王劭[1,2] 程晓霞[1,2] 黄梅清[1,2] 李兆龙[1,2] 郑敏[1,2] 

机构地区：[1]福建省农业科学院畜牧兽医研究所,福州350013 [2]福建省畜禽疫病防治工程技术研究中心,福州350013

出　　处：《中国动物传染病学报》2011年第4期71-75,共5页Chinese Journal of Animal Infectious Diseases

基　　金：福建省科技计划项目(2008N0027);福建省农科院创新团队项目(STIF-Y02)

摘　　要：我国流行的猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus,PRRSV)包括美洲型经典毒株和变异株。本研究应用RT-PCR、直接免疫荧光(direct immunofluorescence asay,DFA)及病毒分离3种检测方法对99份不同类型的猪繁殖与呼吸综合征临床疑似病料和人工感染病料进行检测。结果显示,RT-PCR检测试剂盒敏感性最高,DFA鉴别诊断试剂盒次之,病毒分离最差。DFA与病毒分离、RT-PCR的总符合率分别为92%和85.5%。RT-PCR灵敏性高,可作为PRRSV鉴别诊断的首选方法;DFA鉴别诊断法所需时间较短、特异性好,但需要一定的操作经验;病毒分离敏感性低、操作繁琐,阳性分离需要其它方法验证,不适合于PRRSV的鉴别诊断。Three diagnostic method including the virus isolation and identification （VI）, direct immunofluorescence assay （DFA） and reverse transcriptase polymerase chain reaction （RT-PCR） were employed to detect 99 specimens , parallelly. The results showed that the coincidence between DFA and VI, DFA and RT-PCR were 92.5% and 92.2%, respectively. RT-PCR was the highest sensitivity and specificity, which could be used as the first choice for laboratory differential diagnosis. DFA was high specificity and fast, especially useful for PRRS＇s clinic detecting. The virus isolation＇s procedure was complicated, consumed long time, had a low sensitivity and positive separation needed other methods to identification. Therefore virus isolation was not a suitable test for distinguish detection.

关 键 词：猪繁殖与呼吸综合征病毒美洲型经典毒株 猪繁殖与呼吸综合征病毒变异株 RT-PCR DFA免疫荧光诊断试剂盒 病毒分离 

分 类 号：S852.659.6[农业科学—基础兽医学]