我国九省（市、区）82株嗜肺军团菌血清1型菌株的序列分型  

Sequence-based typing of 82 strains of serotype 1 Legionella pneumophila isolated from 9 provinces in China

作　　者：朱兵清[1] 任红宇[1] 周海健[1] 秦天[1] 邵祝军[1] 

机构地区：[1]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《中华预防医学杂志》2011年第10期890-894,共5页Chinese Journal of Preventive Medicine

基　　金：基金项目：国家“十一五”科技重大专项（2008ZX10004-007,2008ZX10004-008）

摘　　要：目的了解我国环境水中嗜肺军团菌血清1型（Lp1）菌株的序列分型特征，并初步建伊我国军团菌序列分型数据库。方法采用序列分型（SBT）方法，对我国9个省（市、自治区）2005--2008年问环境水中分离的82株如1菌株进行分型，同时采用脉冲场凝胶电泳（PFGE）分型方法对这些菌株进行分型，并采用BioNumerics5．1软件对2种方法的分型结果进行聚类分析和比较。结果82株Lp1菌株分为22种序列（sT）型，其中17种ST型是新序列型，新发现1个等位基因；ST-1型为主要的序列型，在8个省（市、自治区）均有发现，该型菌株占所有菌株的46．3％（38／82）；ST．1、ST．150、ST-154、ST-159、ST-160和ST-630出现于2个以上的分离地点，5个分离位点（B4、B5、B6、S3和S8）发现2种以上ST型；通过聚类分析，15种ST型被分为3个序列群（ST-1序列群、ST-154序列群和ST-149序列群），其余7种ST型没有序列群归类。采用PFGE分型方法，这些菌株可被分为46种带型。SBT和PFGE两种方法结合可将82株菌株分为54种分子型别。两种方法的聚类分析结果具有良好的-致性。结论我国环境水中Lp1菌株具有独特的SBT分布；通过研究，初步建立了我国军团菌序列分型数据库。Objective To analyze the characteristics of Sequence-based Typing （SBT） of the Serotype 1 Legionella pneumophila （ Lp1 ） isolated from environmental water in China, and then create a preliminary database. Methods A total of 82 strains of Lp1 isolated from environmental water in 9 provinces of China between 2005 and 2008 were genotyped by SBT method and Pulsed-field Gel Eleetrophoresis （PFGE） method. The results of the two different typing methods were then compared by cluster analysis, adopting BioNumerics version 5.1 software. Results By SBT method, the 82 strains of Lp1 were divided into 22 ST types, of which 17 new types and one new allele was discovered. The dominant type was ST-1 type,found in 8 provinces, accounting for 46. 3% （38/82） . ST-1, ST-150, ST-154, ST-159, ST-160 and ST-630 types were found in more than 2 isolated-sites; while more than 2 different ST types were found in 5 isolated-sites,as site B4, BS, B6, $3 and $8. In cluster analysis, 15 ST types were grouped into three complexes （ST-1 complex, ST-154 complex and ST-149 complex）; and the other 7 ST types were not assigned complex. By PFGE method ,46 banding patterns were observed. As a result of the combination of the two methods, the 82 isolates strains could be divided into 54 molecular types, which showed a reliable accordance in the cluster analysis between the two methods. Conclusion The SBT of the Lpl in environmental water in China was unique. From the study,a preliminary SBT database was set up.

关 键 词：军团病杆菌 嗜肺 细菌分型技术 电泳 凝胶 脉冲场 

分 类 号：R378[医药卫生—病原生物学]