陕西设施蔬菜根结线虫生防菌株JXC224的分离筛选  

Shanxi facilities vegetables root-knot nematode bio-control strains JXC224 separation screening

作　　者：路鹏鹏[1] 沈卫荣[1] 韩丽萍[1] 沈俭[1] 孙晓宇[1] 门欣[1] 沈讷敏[1] 

机构地区：[1]陕西省微生物研究所,陕西西安710043

出　　处：《广东农业科学》2011年第19期78-80,共3页Guangdong Agricultural Sciences

基　　金：陕西省科学院科技计划项目(2009K03)

摘　　要：几丁质及蛋白质是构成根结线虫体壁和卵壳的主要物质。几丁质酶和蛋白酶活性的高低是评价生防真菌防治潜力的重要生化指标。从陕西省陕北关中陕南根结线虫病害严重的设施蔬菜大棚中采集土样、根结样106份,用分离培养基分离到真菌436株。液体发酵测定分离菌株的几丁质酶活及蛋白酶活,筛选获得几丁质酶活≥0.06 U/mL的菌株11株,蛋白酶活≥100 U/mL的菌株3株。其中JXC224几丁质酶活达0.087 U/mL,蛋白酶活125.8 U/mL,对根结线虫二龄幼虫的侵染率达67%,是一株具有开发潜力的生防菌株,经ITS测序分析,鉴定其为枝顶孢霉(Acremonium sp.)。Chitin and protein is the main composition of root-knot nematode＇ s body wall and egg shell. The activity of chitin enzyme and protease is the important biochemical indexes to evaluate bio-control fungi potential of prevention. 436 strains of fungi were separated from 106 samples which were collected from northern shanxi, guanzhong and south shanxi facilities vegetables suffered from root knot nematode seriously. Among the total strains, 11 strains whose chitin enzyme activity ≥0.06 U/mL and 3 strains whose protease activity ≥ 100 U/mL were screened by liquid fermentation test. JXC224 was the potential strain whose chitin enzyme activity and protease activity was 0.087 U/mL and 125.8 U/mL separately, and the live for root-knot nematode larvae two age infection rate was 67%. JXC224 was identified as A eremonium sp. by ITS strains analysis.

关 键 词：根结线虫 生防菌株 几丁质酶活 蛋白酶活 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]