眼针疗法对大鼠脑缺血再灌注后海马组织脑源性神经营养因子表达的影响  

Effect of Eye Acupuncture on Brain-deprived Neurotrophic Factor Expression in Rat Hippocampus with Ischemia-reperfusion Injury

作　　者：高原[1] 王哲[1] 王莹[1] 王守岩[1] 马贤德[1] 关洪全[1] 王德山[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中国中医药信息杂志》2011年第10期38-39,112,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2007CB512702)

摘　　要：目的观察眼针疗法对大鼠脑缺血再灌注后海马组织脑源性神经营养因子(BDNF)表达的影响,探讨脑保护作用机制。方法线栓法制备SD大鼠大脑中动脉梗死再灌注模型。将SD大鼠随机分为正常对照组、假手术组、模型组、眼针组4组。于再灌注72 h后采用ZeaLonga评分法进行大鼠神经功能缺损评分,采用实时定量PCR方法检测缺血海马组织BDNF mRNA的表达,采用蛋白免疫印迹法检测缺血海马组织BDNF的表达。结果缺血再灌注72 h后,眼针组大鼠神经功能缺损评分显著低于模型组(P<0.05),眼针组大鼠海马组织BDNF mRNA的表达和蛋白的表达较模型组均明显减少(P<0.01)。结论眼针疗法能提高大鼠缺血再灌注后海马组织BDNF表达水平,通过促进神经元的修复发挥脑保护的作用。Objective To investigate the effects of eye acupuncture on brain-derived neurotrophic factor expression（BDNF）,and study its mechnism of cerebral protection from ischemia-reperfusion injury.Methods MCAO model rats established by suture method were randomly divided into the control group,the sham operation group,the model group and the eye-acupuncture group.After reperfusion 72 h,the neurophysical behaviours were accessed by ZeaLonga neurophysical impairment marks.The expression of ischemic hippocampus BDNF mRNA was measured by RT-PCR,and the expression of ischemic hippocampus BDNF protein was detected by Western blot technology.Results After reperfusion 72 h,compared with the model group,the neurologic impairment marks of the eye-acupuncture group decreased obviously（P0.05）.The expressions of BDNF mRNA and protein in rat hippocampus after the eye acupuncture therapy were both obviously decreased（P0.01）.Conclusion Eye acupuncture therapy can increase the expression of BDNF in rat hippocampus with ischemia-reperfusion injury,and protect brain by improving the repair of neurons.

关 键 词：缺血再灌注 脑源性神经营养因子 眼针疗法 海马 大鼠 

分 类 号：R245.329[医药卫生—针灸推拿学]