槲皮素对甲型H1N1流感病毒诱导的A549细胞凋亡效应酶Caspase-3的影响  

Effect of Quercetin on Caspase-3 of A549 Cell Induced by Influenza Virus H1N1

作　　者：万巧凤[1,2] 吴莉[1] 杨美玲[1] 马锐[1] 梁军[1] 顾立刚[2] 

机构地区：[1]宁夏医科大学,宁夏银川750004 [2]北京中医药大学中医药抗病毒教育部重点实验室,北京100029

出　　处：《中国中医药信息杂志》2011年第10期42-44,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：宁夏医科大学校内课题(XM200811)

摘　　要：目的观察银杏叶主要活性成分槲皮素对甲型H1N1流感病毒感染的人肺上皮瘤细胞A549凋亡效应酶Caspase-3的影响。方法采用MTT法测定H1N1毒力、槲皮素细胞毒性作用、槲皮素对H1N1致A549细胞病变的抑制作用。然后用100 TCID50甲型H1N1感染A549 2 h后换用含10 mg/L槲皮素维持液继续培养,于感染后4、12、24、48 h收集细胞,提取细胞总蛋白,采用Western blot方法,应用Image-Pro Plus测定Caspase-3与β-actin灰度值,两者灰度比值为Caspase-3的相对表达量。结果甲型H1N1 A549的TCID50为10-4.75,槲皮素A549的最大无毒浓度为40 mg/L,槲皮素抑制甲型H1N1致A549细胞病变的最小有效浓度为10 mg/L。在甲型H1N1感染A549细胞后4～48 h内,槲皮素可显著抑制Caspase-3蛋白表达,具有明显的抗凋亡作用。结论槲皮素可通过抑制Caspase-3含量或活性而发挥抗病毒感染作用。Objective To investigate the influence of quercetin on Caspase-3 of lung epithelial tumour A549 infected with H1N1.Methods MTT method was adopted to determine H1N1 virulence,quercetin cytotoxicity,inhibitory effect of quercetin on A549 cytopathic with H1N1 cause.H1N1 of 100 TCID50 was used to infect A549 for 2 h and then change quercetin fluid containing 10 mg/L to cultivate.After 4,12,24,48 h,cells were collected to extract total protein.By adopting the Western-blot method and applying Image-Pro Plus,the grayscale value of Caspase-3 and β-actin were determined.The gray ratio was relative expression quantity of Caspase-3.Results The TCID50 of H1N1 to A549 was 10-4.75,the highest non-toxic concentration of quercetin to A549（TC0） was 40 mg/L,the least effective concentration（MEC） of quercetin inhibition to A549 cytopathic with H1N1 cause was 10 mg/L.After the infection of H1N1,quercetin significantly inhibited Caspase-3 expression within 4-48 h,showing that quercetin plays an obvious antiapoptotic role.Conclusion Quercetin can play antiviral infectious functions by inhibiting the content or activity of Caspase-3.

关 键 词：银杏 槲皮素 甲型H1N1流感病毒 A549细胞 CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]