两种紫苏烟和普通烟草芦丁含量的测定和比较  

Determination and comparision of rutin contents in two kinds of zisu tobaccos and ordinary tobacco

作　　者：陈安飞[1] 张金桐[1] 魏治中[1] 

机构地区：[1]山西农业大学,太谷030801

出　　处：《分析仪器》2011年第5期22-25,共4页Analytical Instrumentation

摘　　要：本文采用紫苏烟Q和紫苏烟Q-00001及普通烟草为材料,通过HPLC法测定并比较其中芦丁的含量,目的是找到提取芦丁的新原料,以便于大面积种植推广。HPLC所采用的色谱柱为Apollo C18柱(150mm×4.6mm,5μm),流动相为甲醇︰1.0%磷酸(60︰40,V︰V);柱温25℃;流速0.8mL/min,检测波长为392nm。普通烟草中芦丁含量为0.505%,紫苏烟Q和紫苏烟Q-00001中芦丁含量分别为1.050%和1.267%,后两者芦丁含量为前者的2倍多,其中紫苏烟Q-00001中芦丁含量最高,是一种值得推广种植的用于提取芦丁的新原料。In order to find out new raw materials containing rutin, the rutin contents in zisu tobaccos Q and Q-00001 and ordinary tobacco were determined by HPLC. An Apollo C18 column （150mmX4.6ram, Stem） was employed with CH3 OH ： 1.0% phosphoric acid （60 ： 40, V/V ） as mobile phase, column temperature of 25℃, flow rate of 0.8mL/min, and detection at 392nm. The results showed that in ordinary tobacco the rutin content was 0. 505%, and in zisu tobaccos Q and Q-00001, the rutin contents were 1. 050% and 1. 267%, respectively. The rutin contents in the latter two were twice as much as the former. As zisu to-bacco Q-00001 has the highest rutin content, it is a kind of plant worth to be spreaded.

关 键 词：紫苏烟Q 紫苏烟Q-00001 普通烟草 芦丁 HPLC 

分 类 号：TS47[农业科学—烟草工业]