介孔SiO_2固定化漆酶降解2,4DCP  

Degradation of 2,4-DCP by Immobilized Laccase on Mesoporous Silica

作　　者：杨宇翔[1] 张亚妮[1] 邵建国[2] 陈娅如[1] 

机构地区：[1]华东理工大学化学与分子工程学院,上海200237 [2]扬州大学化学化工学院,江苏扬州225002

出　　处：《同济大学学报（自然科学版）》2011年第9期1324-1328,1393,共6页Journal of Tongji University:Natural Science

基　　金：国家自然科学基金(20971043;20577010)

摘　　要：以M-NH2-SiO2为载体,戊二醛为交联剂制备固定化漆酶,研究2,4-DCP(2,4-二氯苯酚)浓度、溶液酸度、温度对固定化漆酶降解2,4-DCP的影响.结果表明,固定化漆酶降解2,4-DCP最佳条件为:当2,4-DCP质量浓度为5mg.L-1,溶液pH为5.5,温度为30℃时,降解去除率为42.28%.固定化漆酶具有最大活性时的溶液pH值相比于自由酶的最佳pH向碱性偏移;与自由漆酶相比,固定化漆酶有良好的稳定性和重复使用性.Laccase was immobilized on M-NH2-SiO2 with glutaraldehyde as crosslinking agent.The effects on the activity of immobilized laccase,such as concentration of 2,4-DCP（2,4-dichlorophenols）,acidity of solution,temperature were studied.The results show when concentration of 2,4-DCP is 5 mg·L-1,pH of solution is 5.5,and the temperature is at 30℃,the highest removal rate of 2,4-DCP is 42.28%.The results show that the optimal pH for immobilized laccase is higher than the best pH for free enzyme.Compared with the free enzyme,its stability and repetition are considerably improved.

关 键 词：介孔SIO2 固定化漆酶 降解率 重复使用性 

分 类 号：Q814.2[生物学—生物工程] X592[环境科学与工程—环境工程]