康氏木霉PLA_2基因的生物学功能及诱导玉米抗弯孢霉叶斑病作用  

Biological Functions of the Gene PLA_2 of T.koningii and its Induced Resistance Against Curvularia lunata

作　　者：许志华[1,2] 余传金[3,1] 傅科鹤[3,1] 林振亚[3,1] 荆晶[3,1] 王兵[1] 董金皋[2] 陈捷[3,1] 

机构地区：[1]上海交通大学农业与生物学院农业部都市农业(南方)重点开放实验室,上海200240 [2]河北农业大学真菌毒素实验室,河北保定071001 [3]国家玉米产业技术体系研发中心,北京100081

出　　处：《上海交通大学学报（农业科学版）》2011年第4期8-12,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：现代农业产业技术体系(CARS-02);国家自然科学基金项目(30971949);上海市科委重点攻关项目(09391910900)

摘　　要：本研究从康氏木霉T30的cDNA中克隆了PLA2基因,然后用PLA2敲除突变株为材料在玉米接弯孢霉叶斑病菌的条件下研究此基因的生物学功能。敲除突变株P82(ΔPLA2)所分泌的胞外几丁质酶和β-1,3-半乳糖苷酶的酶活要比野生型低。玉米接种弯孢霉叶斑病菌条件下,突变株的生防实验表明其具有较好的诱抗作用。此基因大肠杆菌表达产物在离体玉米叶片上同对照相比产生更大的叶斑。PLA2基因在木霉对抗弯孢霉叶斑病菌同玉米互作可能是一种负调控方式,要对此基因与玉米的非亲和互作还需对此基因互补进行功能验证。In this study,we cloned the gene of PLA2 from cDNA of the wide type T. teoningii T30,and then investigated the biological functions of the PLAe-deletion mutant and the role of PI.Ae demonstrated the interaction between maize and T. koningii after challenged inoculation with Curvularia lunata. The activity of exo-chitinase and 13-1, 3-glucanase of the mutant was a little lower than that of the wild type. Furthermore, the mutant provided a higher control of Curvularia leaf spot on the potted maize in comparison with the wild type strain. Similarly,it had been infiltrated with prokaryotic expression protein of PLA2 got larger lesions on the detached leaves than the controls. Thus we speculated that PI.A2 might be involved in the Trichoderma-induced maize defensive pathway against Curvularia leaf spot in a negativeregulation. The function of PLA2 in detail to non-compatible interaction between Trichoderma spp. and maize need to be further studied through its complemental experiments.

关 键 词：PLA2基因 PLA2敲除突变株 弯孢霉叶斑病 诱导抗性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]