模拟微重力条件下体外成熟卵母细胞的受精能力  

The fertilization of mouse oocytes matured in simulated microgravity

作　　者：吴嫦丽[1,2] 李莉[1] 王芳[1] 吴珍芳[1] 江青艳[1] 张守全[1] 

机构地区：[1]华南农业大学动物科学学院广东省农业动物基因组学与分子育种重点实验室,广州510642 [2]广东医学院基础医学院,湛江524023

出　　处：《安徽农业大学学报》2011年第5期694-697,共4页Journal of Anhui Agricultural University

基　　金：国家973计划(2011CB944202;2010CB945001);国家转基因生物新品种培育重大专项(2008zx08006-004)共同资助

摘　　要：分别对1 g重力和模拟微重力条件下成熟的昆明小鼠卵母细胞进行体外受精试验,研究模拟微重力条件下成熟卵母细胞的受精能力,探讨模拟微重力对卵母细胞成熟的影响。结果显示,1 g重力条件下成熟的小鼠卵母细胞的体外受精率为35.82%,显著高于模拟微重力条件下成熟的卵母细胞的体外受精率。可见,模拟微重力不利于卵母细胞的体外成熟,模拟微重力损伤卵母细胞的受精能力。Mouse oocytes were cultured in microgravity simulated by a rotating wall vessel bioreactor and in Ig gravity in vitro, respectively. We examined the fertilization rate of the mouse ooeytes matured in different gravity conditions. The fertilization rate of mouse oocytes matured in lg gravity was 35.82%, and the oocytes matured in simulated microgravity could not be fertilized in vitro. The results suggest that mouse oocytes maturation and fertilization ability were compromised by the simulated mierogravity in vitro.

关 键 词：模拟微重力 卵母细胞 受精 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]