枯草芽孢杆菌XF-1提取物对11种植物病原菌的抑制作用  

Inhibition effect of Bacillus subtilis XF-1 extract on 11 fungal pathogens

作　　者：贾媛[1] 孙睿揆[2] 吴毅歆[2] 毛自朝[2] 孙海霞[2] 何月秋[1,2] 

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南农业大学农学与生物技术学院,昆明650201

出　　处：《安徽农业大学学报》2011年第5期753-756,共4页Journal of Anhui Agricultural University

基　　金：科技部国际科技合作项目(2009DFA32360);农业部公益性行业(农业)科研专项(201003029);云南省自然科学基金重点项目(2008CC024);云南省科技强省专项(2009EB060)共同资助

摘　　要：采用盐酸和甲醇抽提法,提取了枯草芽孢杆菌XF-1(Bacillus subtilis XF-1)的抑菌物质。以该提取物处理根肿菌孢子2 d,有50%孢子活性被抑制;处理9 d后,孢子萌发相对抑制率始终保持在90%以上。提取物对三七根腐病菌、水稻纹枯病菌、禾谷镰刀菌、魔芋基腐菌、烟草赤星菌生长抑制率分别为71.97%、62.23%、60.96%、60.70%和57.92%。The antagonistic substance,isolated from the culture of Bacillus subtilis XF-1 by a method of hy-drochloric acid precipitation conbined with methanol extraction substance,could inhibit 50% resting spore activity of Plasmodiophora brassicae treated for 2 d and over 90% spore germination treated for 9 d.The inhibition rates were 71.97%,62.23%,60.96%,60.70% and 57.92% of the plant pathogens,i.e.Fusarium solani,Rhizoctonia solani,F.graminearum,F.solani,and Alternaria alternate,respectively.

关 键 词：枯草芽孢杆菌XF-1 根肿菌 拮抗物质 抑菌作用 

分 类 号：S476[农业科学—农业昆虫与害虫防治]