电刺激对废用状态下腓肠肌肌电及酶活性的影响  

Effects of electric stimulation on myoelectricity and enzymatic activity of disused gastrocnemius muscle

作　　者：张浩[1] 杨威[2] 

机构地区：[1]山西医科大学细胞生理学省部共建教育部重点实验室,太原030001 [2]山西医科大学生理学系

出　　处：《山西医科大学学报》2011年第9期738-741,共4页Journal of Shanxi Medical University

基　　金：山西医科大学青年基金资助项目(02201003)

摘　　要：目的本研究旨在观察经皮电刺激对废用状态下肌肉的肌电及酶组织化学的影响。方法用大鼠尾部悬吊法使大鼠下肢去负荷,以建立废用性肌萎缩模型。大鼠随机分为三组:下肢去负荷14 d组(Hu)、电刺激组(Hu+St)及正常对照组(Control)。吊尾期间给予大鼠左侧下肢皮肤低频电刺激(2 Hz,20-25 V)。采用电生理和酶组织化学技术观察腓肠肌肌电图,肌重及肌肉Na+-K+-ATP酶及Ca2+-ATP酶活性。结果下肢去负荷14 d后大鼠腓肠肌肌重,Mmax,Hmax/Mmax,Na+-K+-ATP酶及Ca2+-ATP酶活性均明显降低(P<0.05)。而在下肢去负荷期间给予大鼠左侧下肢电刺激后,以上指标与正常对照组比较均没有统计学差异(P>0.05)。结论以上结果提示,经皮电刺激不仅可对抗废用肌肉的肌重下降,还对肌肉肌电、Na+-K+-ATP酶及Ca2+-ATP酶活性具有保护作用。Objective To explore the effects of transcutaneous electric stimulation（St） on the electromyogram and enzymatic histochemistry of the disused muscle.Methods The disused muscle atrophy model was established by the hindlimb unloading（Hu）.Rats were randomly assigned to 14 d hindlimb unloading group（Hu）,electric stimulation group（Hu＋St） and normal control group.The left hindlimb was transcutaneously given electric stimulation at a low frequency of 2 Hz with voltage of 20-25 V during the period of hindlimb unloading in Hu＋St rats.Using electrophysiological and histochemical techniques,the electromyogram,muscular weight,Na＋-K＋-ATPase and Ca2＋-ATPase activities of gastrocnemius muscle were observed.Results After 14 d hindlimb unloading,wet weight,mean maximal motor response（Mmax）,ratio of maximal Hoffmann reflex and Mmax（Hmax/Mmax）,Na＋-K＋-ATPase and Ca2＋-ATPase activities of gastrocnemius muscle were all significantly decreased（P0.05）.While all the above indices for Hu＋St rats were not statistically different from those of the control rats（P0.05）.Conclusion The transcutaneous electric stimulation can not only counteract the decrease of muscle wet weight,but also protect electrophysiology and enzymatic metabolism activities of disused muscle.

关 键 词：下肢去负荷 电刺激 肌萎缩 腓肠肌 

分 类 号：R337.5[医药卫生—人体生理学]