活血颗粒质量标准研究  

Study on the quality standard of Huoxue granules

作　　者：张志勇[1] 李艳丽[2] 高翔宇[1] 刘恩荔[1] 

机构地区：[1]山西医科大学药学院中药学教研室,太原030001 [2]山西生物应用职业技术学院生物制药工程系

出　　处：《山西医科大学学报》2011年第9期745-748,共4页Journal of Shanxi Medical University

摘　　要：目的建立活血颗粒的质量标准。方法采用薄层色谱法对方中白芍、柴胡、白芷、穿山甲等药味进行定性鉴别;采用高效液相色谱法,对方中白芍中的芍药苷进行含量测定,以十八烷基硅烷键合硅胶为填充剂;以甲醇-水(29∶71)为流动相;检测波长为230 nm。结果本品薄层色谱斑点清晰、专属性强。芍药苷含量测定线性范围为0.123 5-0.617 5μg;平均回收率分为97.85%;RSD值为2.34%。结论定性鉴别和含量测定方法准确可行,重复性好,可有效地控制活血颗粒的质量,建立了活血颗粒的质量标准。Objective To establish a quality standard of Huoxue granules.Methods Radix Paeoniae Alba,Radix Bupleuri,Radixngelicae Dahuricae,Semen Vaccariae in Huoxue granules were qualitatively identified by thin layer chromatography（TLC）.The content of paeoniflorin in Huoxue granules was determined by high performance liquid chromatography（HPLC）.ODS column was used with methanol-water（29∶ 71） as mobile phase.The UV detector was set at 230 nm.Results The average recovery was 97.85%,and RSD was 2.34%.The calibration curve was linear in the range of 0.123 5-0.617 5 μg（r=0.999 9）.Conclusion The methods are accurate and reliable,and could be used for the quality control of the Huoxue granules.A new quality standard of Huoxue granules is successfully established.

关 键 词：活血颗粒 薄层色谱法 芍药苷 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学]