基于中空纤维液相微萃取的黄酮类化合物测定  

Determination of contents of flavonoids in Platycladus orientalis based on hollow fiber liquid phase microextraction

作　　者：全红[1] 张茜[1] 陈璇[1] 白小红[1] 

机构地区：[1]山西医科大学药学院药物分析教研室,太原030001

出　　处：《山西医科大学学报》2011年第9期749-754,共6页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(81041084);山西省自然科学基金资助项目(2011011035-2)

摘　　要：目的建立中空纤维液相微萃取(HFLPME)-高效液相色谱法(HPLC)测定中药侧柏中黄酮类化合物含量。方法以聚偏氟乙烯为有机溶剂载体,正己醇为接受相,10-5 mol/L HCl为供相,在1 500 r/min搅拌下萃取45 min,萃取结束后,蒸干接受相中有机溶剂,用50μl甲醇溶解后进行HPLC分析。结果分析物二氢杨梅素、杨梅素、漆黄素、槲皮素、柚皮素、山柰素、异鼠李素和白杨素在0.01-10.0μg/ml范围内线性关系良好;检出限为0.01-1.0 ng/ml;平均浓缩因子在5.4-30.6倍之间;中药侧柏中分析物的平均回收率为97.5%。结论 HFLPME操作简单、快速、有机溶剂用量少、富集倍数高、对测定中药中微量或痕量黄酮类有效成分具有重要的意义。Objective To establish a hollow fiber liquid phase microextraction（HFLPME） coupled with high performance liquid chromatography（HPLC） for determining the contents of the flavonoids in Platycladus orientalis.Methods The analytes were extracted under 1 500 r/min agitation for 45 min with polyvinylidene difluoride as the organic solvent supporter,10-5 mol/L HCl as the donor phase,and n-hexanol as the acceptor phase.After extraction,the organic solvent in the acceptor phase was dried,and then dissolved with 50 μl methanol.The analytes were determined by HPLC.Results The analytes showed a good linear relationship by HFLPME-HPLC in the range of 0.01-10.00 μg/ml.The limits of detection were 0.01-1.0 ng/ml.The enrichment factors of flavonoids ingredients ranged from 5.4 to 30.6.The average recovery of analytes in Platycladus orientalis was 97.5%.Conclusion The method is simple,rapid,and effective.It has a great significance on the analysis of trace flavonoid compounds in traditional Chinese medicine.

关 键 词：中空纤维液相微萃取 浓缩因子 侧柏 黄酮类化合物 

分 类 号：R917[医药卫生—药物分析学]