SD大鼠胃窦平滑肌细胞分离方法的改进  

Isolation of gastric antrum smooth muscle cells from Sprague-Dawley rats

作　　者：谢燕东[1] 杨琦[1] 王景杰[1] 黄裕新[1] 

机构地区：[1]第四军医大学唐都医院消化内科,西安710038

出　　处：《山西医科大学学报》2011年第9期772-774,776,共4页Journal of Shanxi Medical University

基　　金：国家自然科学基金资助项目(30801487)

摘　　要：目的改进SD大鼠胃窦平滑肌细胞的分离方法,为胃肠道疾病的研究提供技术平台。方法Ⅱ型胶原酶消化法急性分离SD大鼠胃窦平滑肌细胞,制成细胞悬液,台盼蓝检测细胞活力,显微镜下观察记录细胞收缩长度。结果 1%Ⅱ型胶原酶消化法急性分离可获得细胞膜完整、生物活性状态良好的单个胃窦平滑肌细胞,该细胞符合胃肠道疾病实验研究的要求,可用于相关实验研究。结论酶消化急性分离法是获得单个胃肠道平滑肌细胞的一种简便、高效、易操作的方法。Objective To explore a series of convenient methods for better isolating the gastric antrum smooth muscle cells from Sprague-Dawley rats.Methods The smooth muscle cells were isolated by collagenase digestion of typeⅡcollagen from the gastric antrum of rats for making a cell suspension.Then cell vitality was detected with trypan blue,and contraction length of cell was observed and recorded under a microscope.Results The single gastric antrum smooth muscle cell was acutely dissociated by collagenase with complete physiological functions,which can be used in gastroenteropathy experiments.Conclusion Isolation of gastric antrum smooth muscle cells by collagenase is convenient,effective and reliable to obtain the single gastric antrum smooth muscle cells.

关 键 词：平滑肌细胞 分离 大鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]