Electrochemical mechanism of rusticyanin (Rus.) isolated from A.ferrooxidans measured by Rus.-ZnS-QDs/L-Cys/Au electrode  

作　　者：孙静 余润兰 苗雷 钟代立 刘杰 顾帼华 

机构地区：[1]Key Laboratory of Biometallurgy of Ministry of Education,School of Minerals Processing and Bioengineering,Central South University

出　　处：《Journal of Central South University》2011年第5期1389-1394,共6页中南大学学报（英文版）

基　　金：Project(2010CB630903) supported by the National Basic Research Program of China;Project(50621063) supported by the National Natural Science Foundation of China

摘　　要：Electrochimcal behaviors of rusticyanin （Rus.） isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode. The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction. The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19-43 ℃. The reduction ability of the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8. Fe^2＋ ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials （φp） markedly move negatively.Electrochimcal behaviors of rusticyanin (Rus.) isolated from Acidithiobacillus ferrooxidans were investigated through Rus.-ZnS-QDs/L-Cys/Au electrode.The cyclic voltammetric results indicate that rusticyanin immobilized on the surface of Rus.-ZnS-QDs/L-Cys/Au electrode can undergo a direct quasi-reversible electrochemical reaction.The immobilized rusticyanin is not denatured and still retains its activity in the temperature range of 19-43 °C.The reduction ability of the protein increases and its oxidation ability becomes weak with the increase of pH from 6.0 to 7.8.Fe2+ ions in the solution can promote the electron transfer kinetics of the immobilized rusticyanin and make its peak potentials (φp) markedly move negatively.

关 键 词：rusticyanin acidithiobacillusferrooxidans cyclic voltammetry ELECTROCHEMISTRY 

分 类 号：O657.1[理学—分析化学]