重组人骨形态发生蛋白2-肝素-人工骨复合材料的骨诱导活性  

Bioactivities of recombinant human bone morphogenetic protein 2-heparin artificial bone composite materials

作　　者：周智优[1] 何水连[1] 孙莉[1] 王丁丁[2] 汪炬[1] 

机构地区：[1]暨南大学生命科学技术学院生物工程研究所,广东省广州市510632 [2]广东药学院生命科学与生物制药学院,广东省广州市510006

出　　处：《中国组织工程研究与临床康复》2011年第34期6276-6280,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：广东省科技攻关项目(2009B030801237);广东省教育部科技部企业科技特派员行动计划项目(2009B090600134)~~

摘　　要：背景:已有将重组人骨形态发生蛋白2应用于骨再生及修复的报道,但由于其在生物体内半衰期短而导致诱导骨形成的能力受到限制。目的:制备具有较好缓释效果的重组人骨形态发生蛋白2-肝素-人工骨复合材料,并检测其缓释性能及骨诱导活性。方法:通过高效液相色谱法检测重组人骨形态发生蛋白2-肝素复合物对于酶解的保护作用。将重组人骨形态发生蛋白2与肝素溶液混匀后复合于人工骨材料表面,ELISA方法检测其体外释药性质,茜素红染色法检测其诱导成骨细胞的能力,应用小鼠体内实验评价其异位骨诱导能力。结果与结论:成功制备了具有良好缓释效果的重组人骨形态发生蛋白2-肝素-人工骨复合材料,具有较强的诱导骨钙蛋白及异位骨形成能力。BACKGROUND：Recombinant human bone morphogenetic protein 2（rhBMP-2） has been applied to bone regeneration and repairing,but its ability to induce bone formation is limited because of its short half-life in vivo.OBJECTIVE：To prepare rhBMP-2-heparin composite materials and to evaluate rhBMP-2 sustained delivery and osteoinductive effects.METHODS：The effect of heparin on protecting rhBMP-2 from enzyme digestion was assayed by high-performance liquid chromatography.The materials were combined with rhBMP-2-heparin to produce rhBMP-2-heparin artificial bone composite materials,detecting the rhBMP-2 release effect by ELISA.Alizarin red staining was used to detect osteogenic ability.The ability to induce ectopic bone was detected through an in vivo mouse experiment.RESULTS AND CONCLUSION：It is successful to prepare rhBMP-2 artificial composite materials with good sustained release effect of rhBMP-2,which can effectively induce osteocalcin and ectopic bone formation.

关 键 词：重组人骨形态发生蛋白2 肝素 人工骨复合材料 缓释 骨形成 异位骨 

分 类 号：R318[医药卫生—生物医学工程]