多孔丝素材料组织相容性的初步研究  

A preliminary study on the histocompatibility of silk fibroin

作　　者：陆艳[1] 赵霞[1] 邵正中[2] 曹正兵[2] 蔡丽慧[1] 

机构地区：[1]复旦大学附属华山医院耳鼻咽喉-头颈外科,上海市200040 [2]复旦大学高分子系,上海市200433

出　　处：《中国组织工程研究与临床康复》2011年第34期6364-6367,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金面上项目(30772400);上海医学院青年骨干科研启动基金(09L-12)(B类)(部分资助)~~

摘　　要：背景:丝素蛋白支架材料被植入生物体内后会发生降解且无法完全与宿主组织分离,这类材料生物相容性的研究大多为体外实验,其体内的组织相容性和降解过程的研究结果仍不充分。目的:初步观察多孔丝素材料的体内组织相容性。方法:将多孔丝素支架埋藏于SD大鼠背部皮下,术后2,4,6,8周分别取材,对伤口局部及材料情况大体观察,然后材料切片苏木精-伊红染色行组织学观察。结果与结论:动物伤口愈合良好,多孔丝素表面形成极薄的纤维包裹,周围组织反应轻微。组织切片见炎细胞浸润,以巨噬细胞为主,支架材料边缘孔隙内有成纤维细胞和毛细血管长入。8周时材料边缘部分可见支架结构崩解现象,而材料内部变化不大。结果显示组织细胞可以沿多孔丝素支架表面贴附生长,提示支架材料具有较好的组织相容性。BACKGROUND：As one kind of naturally occurring polymers,silk fibroin has been widely used in tissue engeering research,which can be made into different forms for application. Scientists find many difficulties in histocompatibility study of silk fibroin,because it is made of protein and biodegradable. And there are few reports on the study on histocompatibility of silk fibroin. OBJECTIVE：To perform a preliminary in vivo study on the histocompatibility of silk fibroin. METHODS：Porous silk fibroin scaffolds were buried into the subcutaneous part of the back of SD rats. At 4,6,8 weeks after operation,general observation and histological examination were performed. RESULTS AND CONCLUSION：The wounds healed up well after operation. Only thin connective tissue wrap was found on silk fibroin scaffold. Macrophages were observed in tissue section,and fibroblasts and capillary vessels could grow into the pore spaces. We found the border of the scaffolds break down at 8 weeks postoperatively,while the inside part remained as it was. Porous silk fibroin scaffold shows good histocytes attachment and has good histocompatibility. More study should be made on the mechanism and product of degradation in vivo.

关 键 词：丝素蛋白 多孔支架 组织工程 组织相容性 体内实验 

分 类 号：R318[医药卫生—生物医学工程]