成骨细胞表面不同神经肽受体的表达  

Expression of different neuropeptide receptors on osteoblast surface

作　　者：张学敏 马文辉[2] 时述山[3] 张卫平[2] 冯志军[2] 李亚非[3] 贾恒川[2] 胡兴茂[2] 郑晓华 

机构地区：[1]河北省国防建医院,河北省石家庄市050081 [2]解放军白求恩国际和平医院骨科,河北省石家庄市050082 [3]解放军北京军区总医院骨科,北京市100072

出　　处：《中国组织工程研究与临床康复》2011年第33期6107-6110,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:大量研究表明神经因素可调节骨代谢,迄今为止已发现5种神经肽参与骨代谢过程。目的:观察正常人成骨细胞表面不同神经肽受体的表达。方法:分别以降钙素基因相关肽、酪氨酸羟化酶、P物质、神经肽Y的单克隆抗体进行免疫组化染色,观察各种肽类受体在成骨细胞表面的表达情况。另外,利用计算机图像分析系统对染色灰度进行半定量分析。结果与结论:正常人成骨细胞表面有神经肽Y、P物质、酪氨酸羟化酶、降钙素基因相关肽受体表达,这些因子可以通过与相应的受体结合影响细胞生物学特性。不同因子免疫组化染色的灰度值由小到大依次为神经肽Y、降钙素基因相关肽、酪氨酸羟化酶、P物质,神经肽是成骨细胞活性的重要调节因子。BACKGROUND：A large number of studies have demonstrated that neuropeptide can regulate bone metabolism.There have been five neuropeptides found to participate in the process of bone metabolism.OBJECTIVE：To observe the expression of different neuropeptides on the surface of osteoblasts of healthy adults.METHODS：Calcitonin gene-related peptide,tyrosine hydroxylase,P substance,neuropeptide Y monoclonal antibodies were used for immunohistochemical staining to observe the expression of various neuropeptide receptors on the surface of osteoblasts.In addition,semi-quantitative analysis of staining gray scale was performed through the use of computer image analysis system.RESULTS AND CONCLUSION：Neuropeptide Y,P substance and calcitonin gene-related peptide receptor expression was observed on the surface of osteoblasts of healthy adults and these factors influence cellular biological characteristics after binding with corresponding receptors.The gray value from low to high was in the following sequence,neuropeptide Y,calcitonin gene-related peptide,tyrosine hydroxylase,P substance.Results showed that neuropeptide is an important regulatory factor of biological activity of osteoblasts.

关 键 词：神经肽 受体 成骨细胞 免疫组织化学 因子 

分 类 号：R318[医药卫生—生物医学工程]