静磁场作用下金属离子对成骨细胞的毒性  

Static magnetic field reduces the toxic effect of metal ions on osteoblasts

作　　者：周通华[1] 戴闽[1] 程细高[1] 詹平[1] 熊皞[1] 邹文楠[2] 严小青[2] 

机构地区：[1]南昌大学第一附属医院骨二科,江西省南昌市330006 [2]南昌大学建筑工程学院工程力学实验中心,江西省南昌市330046

出　　处：《中国组织工程研究与临床康复》2011年第35期6567-6570,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金资助项目(30760255);南昌大学学科交叉基金(Z03722)~~

摘　　要：背景:体内体外实验研究证明,静磁场具有促进成骨细胞分化及骨形成的作用。目的:观察静磁场作用下金属离子对成骨细胞毒性的影响。方法:将CoCl2粉末与CrCl3粉末溶于无菌注射用水配制成CoCl2溶液和CrCl3溶液,将金属离子Co2+、Cr3+分别与小鼠颅骨成骨细胞(MC3T3-E1)在不同的磁场强度(1,10,100mT)下共培养。实验分4组:设Co2++Cr3+组(对照组)和Co2++Cr3+分别+1,10,100mT组。结果与结论:恒磁场作用下的成骨细胞呈现更加成熟的形态学特征,Co2+、Cr3+金属离子对成骨细胞MC3T3的毒性明显降低;同时G2M(分裂期)分布比例明显增加,细胞停滞在G0G1(休眠期)的比率明显减少;与对照组相比,不同场强磁场加载后,成骨细胞碱性磷酸酶活性明显增强(P<0.05)。提示一定强度的静磁场可拮抗Co2+、Cr3+金属离子对成骨细胞的毒性作用。BACKGROUND：Static magnetic field can promote the proliferation,differentiation and functional activity of osteoblasts.OBJECTIVE：To investigate the toxic effect of metal ions Co2＋,Cr3＋ on osteoblasts under the static magnetic field.METHODS：CoCl2 powder and CrCl3 powder were dissolved in the asepsis injecting water.Mouse calvarial osteoblasts（MC3T3-E1） were cultured with Co2＋ and Cr3＋ ions in different magnetic field of 1,10,100 mT.Cells only cultured with Co2＋ and Cr3＋ ions were taken as control group.RESULTS AND CONCLUSION：Under the static magnetic field,osteoblasts showed more mature morphological features;the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts reduced significantly;G2M（dividing phase） distribution ratio increased and G0G1（dormancy stage） distribution ratio decreased;the A value tested by alkaline phosphatase activity in the three experiment groups up-regulated obviously.The static magnetic field in certain intensity could antagonize the toxic effect of metal ions Co2＋ and Cr3＋ on osteoblasts.

关 键 词：静磁场 成骨细胞 金属离子 无菌性松动 体外实验 

分 类 号：R318[医药卫生—生物医学工程]