白细胞介素16基因多态性与广西地区汉族女性膝关节原发性骨性关节炎的易感关联  

Association of the interleukin-16 gene polymorphisms with primary knee osteoarthritis susceptibility in female population of Guangxi Zhuang Autonomous Region

作　　者：罗世兴[1] 秦雪[2] 赵劲民[1] 苏伟[1] 邓燕[2] 

机构地区：[1]广西医科大学第一附属医院创伤骨科手外科,广西壮族自治区南宁市530021 [2]广西医科大学第一附属医院检验科,广西壮族自治区南宁市530021

出　　处：《中国组织工程研究与临床康复》2011年第35期6647-6650,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前的基因研究表明,膝关节原发性骨性关节炎的发病风险与白细胞介素类基因有关联性。目的:观察白细胞介素16基因多态性与女性膝关节原发性骨性关节炎的相关性。方法:收集71例Kellgren/Lawrence评分大于2分的膝关节原发性骨性关节炎患者及85例健康对照的广西汉族女性的静脉血标本,采用聚合酶链反应-限制性片段长度多态性技术对两组白细胞介素16的rs 11556218,rs 4072111,rs 4778889多态位点进行基因分型,计算两组基因型和等位基因的分布差异,分析白细胞介素16基因多态性与膝骨性关节炎的易感关联。结果与结论:两组3个SNP位点的各基因频数在两组中的分布符合Hardy-Weinberg平衡,等位基因比较差异无显著性意义。单个位点显性模式非条件Logistic回归显示,在rs 11556218的3种基因型的比较中,T/G基因型较T/T基因型的膝原发性骨性关节炎的发病风险显著减低(OR=0.41,95%CI:0.21～0.83,P=0.02);在rs 4072111中,C/T基因型比C/C基因型的发病风险显著增加(OR=2.36,95%CI:1.19～4.68,P=0.03)。结果证实,广西地区汉族女性人群白细胞介素16的遗传变异与膝关节原发性骨性关节炎的易感性关联。BACKGROUND：Current genetic researches show that the risk factors for knee osteoarthritis（OA） mostly related to interleukin（IL） 1,6,10 and other interleukin genes.OBJECTIVE：To investigate whether the IL-16 gene polymorphisms is related to genetic susceptibility to primary OA in female.METHODS：Blood samples were obtained from 71 primary OA patients defined by a Kellgren-Lawrence score of 2 or greater,and 85 controls of Guangxi Han female population.The polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to detect IL-16 gene（rs11556218,rs4072111,rs4778889） distribution between the two groups.Genotype and allele frequencies were calculated by SPSS,and Hardy-Weinberg equilibriums and non-conditional logistic regression was performed to evaluate the association between OA susceptibility and genotype using the SNPstats.RESULTS AND CONCLUSION：All genotype distributions of three single-nucleotide polymorphisms between the case and control group met Hardy-Weinberg equilibrium criteria（P0.05）.The results of non-conditional logistic regression analysis in codominant model showed that the T/T genotype of the rs11556218 might be a protective factor（OR=0.41,95%CI=0.21-0.83,P=0.02） and the C/T genotype of the rs4072111 might be a risk factor（OR=2.36,95%CI=1.19-4.68,P=0.03）.It is indicated that the genetic polymorphisms of IL-16 gene may be associated with OA susceptibility in the Guangxi Han female population.

关 键 词：膝关节骨性关节炎 白细胞介素16 基因 单核苷酸多态性 组织构建 

分 类 号：R318[医药卫生—生物医学工程]