营养元素对三角褐指藻生长和脂类积累的影响(英文)  

Effect of Nutrient Elements on Growth and Lipid Accumulation of Phaeodactylum tricornutum

作　　者：陈昱[1] 邱昱晶[2] 张维[1] 吕素娟[2] 刘天中[1] 

机构地区：[1]中国科学院青岛生物能源与过程研究所生物燃料重点实验室,山东青岛266101 [2]中国海洋大学食品科学与工程学院,山东青岛266003

出　　处：《生物质化学工程》2011年第5期1-6,共6页Biomass Chemical Engineering

基　　金：山东省科技攻关项目资助(2008GG20007002);中国科学院太阳能计划资助(KGCX2-YW-374-4)

摘　　要：研究了不同营养元素(氮、磷、硅、铁)在不同培养浓度条件下,对三角褐指藻生长以及细胞脂类积累的影响作用。结果显示:藻细胞生长随氮含量的增加而增加,但超过一定浓度后作用不明显,最适合三角褐指藻生长的氮含量为1.76 mmol/L。缺氮和低氮(0.22～0.44 mmol/L)培养能够明显提高藻细胞脂类含量。缺磷培养能有效促进藻株脂类积累,但是藻细胞生长受到明显抑制。缺硅导致藻细胞的生长减缓,但是对藻细胞的油脂积累影响不显著。在缺铁和高铁(0.048 mmol/L)条件下,藻的总脂含量均高于正常的培养条件。实验证明通过两步培养的方法,先在营养充足的培养基中进行藻细胞生长,然后将藻细胞转移到海水中培养,可以明显提高藻细胞的油脂含量。最终本实验确定海水为诱导三角褐指藻脂类积累的最佳诱导培养基。Effects of nutrient elements,such as nitnogen,phosphor,silicon and iron,on growth and lipid accumulation of Phaeodactylum tricornutum were investigated by Nile-red fluorescence method.The results showed that higher nitrogen content would enhance the growth of algal cell and the optimal concentration is 1.76 mmol/L.Nitrogen deficiencies（0.22-0.44 mmol/L） induced the lipid synthesis remarkably.Phosphor deficiencies inhibited the growth of cells,but greatly induced the lipid synthesis.Silicon deficiencies caused a slow down on cell growth,but little effect on lipid accumulation.Either iron deficiency or iron excess（0.048 mmol/L） would enhance the lipid accumulation in P.tricornutum cells.In two-stage culture method,the first step is to grow cell in the nutrients-abundant medium and then transfer it to nitrogen/phosphor-deficienct medium for lipid induction in the second stage.The fat content of algal cell can be improved significantly by two-step culture method.Seawater is feasible to be used as a good lipid induction medium for P.tricornutum.

关 键 词：三角褐指藻 营养元素 脂类积累 荧光光谱测定法 

分 类 号：Q945[生物学—植物学]