茉莉酸诱导侧根形成缺陷突变体asa1-1抑制子(soa)的鉴定与遗传分析  

Genetic screening and analysis of suppressors of asa1-1(soa) defective in jasmonate-mediated lateral root formation in Arabidopsis

作　　者：李延安[1,2] 祁林林[2] 孙加强[2] 刘宏宇[1] 李传友[2] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]中国科学院遗传与发育生物学研究所植物基因组学国家重点实验室,北京100101

出　　处：《遗传》2011年第9期1003-1010,共8页Hereditas（Beijing)

基　　金：国家自然科学基金重大研究计划项目(编号:90717007)资助

摘　　要：外源茉莉酸处理野生型拟南芥能够促进侧根的形成,而在asa1-1突变体中茉莉酸抑制侧根的形成,这与在该突变体背景下茉莉酸显著降低PIN2蛋白水平密切相关。为了进一步研究茉莉酸诱导PIN2蛋白水平下调的分子机制,文章采用正向遗传学的方法筛选asa1-1抑制子soa,期望获得茉莉酸处理后侧根发育恢复的突变体。通过筛选鉴定获得2个突变体:soa563和soa856。这2个突变体在10μmol/L茉莉酸甲酯处理条件下都能够恢复侧根发育,而且茉莉酸处理后PIN2蛋白水平降低的现象在soa563中被完全抑制,在soa856中被部分抑制。这些结果表明这两个突变基因可能影响了茉莉酸调控的PIN2蛋白水平下调途径,并且参于了茉莉酸对侧根发生的调控。对这两个基因的分离和功能研究将为阐明茉莉酸与生长素互作调控侧根发生的分子机制提供新的知识积累。It has been shown that jasmonate modulates the lateral root development through crosstalk with auxin in Arabidopsis thaliana.Exogenous application of jasmonate stimulates lateral root formation in wild type but inhibits lateral root formation in asa1-1.Our previous work has demonstrated that the lateral root formation defect of asa1-1 is co-related with jasmonte effect on PIN2 protein levels.To further elucidate the molecular mechanisms underlying jasmonate-mediated reduction of plasma membrane（PM）-resident PIN2 abundance,we have conducted a genetic screen to identify suppressors of asa1-1（soa）,which showed lateral root formation in the presence of jasmonate.Here,we described the basic characteri-zation of soa563 and soa856.We showed that both soa563 and soa856 displayed restored lateral root formation in response to exogenous jasmonate.In addition,jasmonate-induced PIN2：GFP reduction was blocked in these two mutants.Our on-going effort to identify genes defined by these mutants promise to shed new light on the understanding of the molecular mechanisms controlling jasmonate-mediated regulation of PIN2 protein trafficking and turnover.

关 键 词：拟南芥 茉莉酸 生长素 PIN2蛋白 侧根形成 

分 类 号：Q946[生物学—植物学]