登革病毒2型诱导人血管内皮细胞株EAhy926自噬的研究  

DV2-induced Autophagy in Human Endothelial Cell Line EAhy926

作　　者：李颖[1] 郑毅涛[1] 齐一鸣[1] 黄俊琪[1] 

机构地区：[1]中山大学中山医学院免疫教研室//教育部热带病防治研究重点实验室,广东广州510080

出　　处：《中山大学学报（医学科学版）》2011年第4期433-436,共4页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(30872350);广东省自然科学基金(8151008901000017);广东省科技计划项目(2007B031516008;2010B050700008);广州市科技计划项目(2008Z1-E221);广东省软科学研究计划(2008B060600051);高校基本科研业务费中山大学青年教师培育项目(10YKPY31)

摘　　要：【目的】探讨登革病毒2型(DV2)感染人静脉血管内皮细胞株EAhy926对细胞自噬的影响。【方法】同一批EAhy926细胞分成对照组和感染组,感染组在DV2感染EAhy926细胞后12、24、36、48h分别收集细胞,用流式细胞仪检测发生自噬的细胞百分率与酸性自噬泡的平均荧光密度,对照组相应时间点收集细胞做同样处理作为对照。实验重复5次,用方差分析和配对t检验进行统计分析。【结果】感染组4个时间点自噬的细胞百分率和酸性自噬泡的平均荧光密度较对照组相应时间点均有升高(P<0.05)。48h对照组和感染组自噬细胞的百分率和酸性自噬泡平均荧光密度差异最明显。对照组4个时间点自噬细胞百分率(%)分别为:70±7,72±6,71±8和69±10;感染组则为75±3,78±3,77±5,80±7。对照组4个时间点酸性自噬泡平均荧光密度分别为:36.4±3.4,37.0±3.3,35.8±2.3,34.4±3.2,感染组则为41.1±2.3,45.0±4.8,41.3±3.3,44.5±7.5。【结论】DV2能诱导EAhy926细胞发生自噬,但细胞发生自噬的相关信号传导通路还有待进一步研究。[Objective] To investigate the DV2（Dengue virus type 2）-induced autophagy in the human endothelial cell line EAhy926.[Methods] Flow cytometric analysis was used to detect the percentage of autophagic cells of EAhy926 and the mean fluorescent intensity（MFI） of autophagic vacuoles after DV2 infection for various time periods（12 h,24 h,36 h,and 48 h）,at the same time,the Eahy926 cells in control groups were collected for detection.All experiments were repeated for 5 times.The variance analysis and the t test were used for analysis.Values were considered statistically significant at P 0.05.[Results] Compared with the control groups,the percentage of autophagic cells of EAhy926 and the MFI of the autophagic vacuoles increased in DV2 infection groups（P 0.05）.The differential value between control groups and DV2 infection groups was most significant in 48 h.In different times（12 h,24 h,36 h,and 48 h）,there were（70 ± 7）%,（72 ± 6）%,（71 ± 8）%,and（69 ± 10）% of autophagic cells in control groups respectively,while in DV2 infection groups,there were（75 ± 3）%,（78 ± 3）%,（77 ± 5）%,and（80 ± 7）% of autophagic cells,respectively.Accordingly,the MFI of autophagic vacuoles in control groups was 36.4 ± 3.4,37.0 ± 3.3,35.8 ± 2.3,and 34.4 ± 3.2 in the four timepoints respectively,while in DV2 infection groups,the MFI of autophagic vacuoles was 41.1 ± 2.3,45.0 ± 4.8,41.3 ± 3.3,and 44.5 ± 7.5,respectively.[Conclusions] DV2 can induce the autophagy of EAhy926.

关 键 词：登革病毒 自噬 EAhy926 酸性自噬泡 

分 类 号：R373.33[医药卫生—病原生物学]