妊娠期高血压患者外周血中的脂代谢基因差异表达  

Differential Lipid Metabolism-related Genes in Patients with Hypertensive Disorder Complicating Pregnancy

作　　者：徐丽南[1] 王子莲[1] 何科[1] 张颖[1] 蔡坚[1] 杨建波[1] 周灿权[1] 

机构地区：[1]中山大学附属第一医院妇产科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2011年第4期467-471,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2010B031600313)

摘　　要：【目的】研究妊娠期高血压疾病患者在孕16～20周外周血白细胞的基因表达谱,寻找妊娠期高血压疾病患者中孕早期外周血白细胞的差异表达基因,并选取异常脂代谢基因进行认证。【方法】我院2008年8月至2008年12月间在我院产检且经B超确定孕期为16～20周的初产妇共800例,随访至妊娠结束,将最终发展为子痫前期的6例患者为研究组,40例正常妊娠妇女为对照组,应用人类全基因组cDNA单荧光芯片检测两组孕妇在妊娠16～20周时外周血白细胞差异基因表达谱;在差异表达基因中选取氧化修饰低密度脂蛋白(oxLDL)使用酶联免疫吸附试验法(ELISA)检测血浆浓度;血凝素样氧化低密度脂蛋白受体-1(LOX-1)及载脂蛋白A5(APOA5)进行RT-PCR测定。【结果】研究组和对照组存在差异表达基因,筛选得到983个差异表达基因,其中719个基因表达上调,264个基因表达下调;研究组oxLDL水平、LOX-1水平较正常组明显升高(P<0.05);研究组APOA5水平较正常组明显降低(P<0.05);oxLDL水平与LOX-1mRNA表达水平呈正相关(r为0.91,P<0.05)。【结论】妊娠期高血压疾病患者外周血白细胞的脂代谢基因oxLDL、LOX-1及APOA5基因表达在妊娠16～20周时与正常妊娠存在明显差异。[Objective] The detection of peripheral leucocytes expression profile between normal pregnancy and preeclampsia at 16-20 gestation weeks,and compare the gene expression difference between them with using DNA microarray technique and human genome cDNA chip.And to seek the factors inducing disorders of lipid metabolism in preeclampsia by testing the difference of lipid metabolism-related genes expression.[Methods] A cohort of nulliparous women was recruited.Preeclampsia developed in 6 patients with severe preeclampsia（research group） were diagnosed according to the criteria（Obstetrics and Gynecology,the Sixth Edition）,without complications of internal medicine and other complications of pregnancy）,40 normotensive controls women were matched（control group）.To get detection of peripheral leucocytes expression profile at 16-20 gestation weeks,and compare the gene expression difference between two groups using DNA microarray technique and human genome cDNA chip.To detect the plasma levels of oxidized low density lipoprotein（ox-LDL） by ELISA and the deposition of Lectin like oxidized low density lipoprotein receptor-l（LOX-1） and apolipoproteins A5（APOA5） by RT-PCR.[Results] The results of microarray analysis of mRNA in peripheral leucocytes between two groups：there were 983 differentially expressed genes in gene chip,in which 719 up-regulating genes and 264 down-regulating genes.The plasma levels of ox-LDL and LOX-1 were significant higher in study group than that in control group（P 0.05）,the level of APOA5 were significant lower in study group than that in control group（P 0.05）,the level of LOX-1 was positively correlated with the plasma concentration ox-LDL（R = 0.91,P 0.05）.[Conclusion] oxLDL,LOX-1,and APOA5 gene expression of peripheral leucocytes in pre-eclampsia patients is significantly different from that of normal pregnancy.

关 键 词：妊娠期高血压疾病 子痫前期 基因芯片 OXLDL LOX-1 APOA5 

分 类 号：R714.246[医药卫生—妇产科学]