鞘内注射蛙皮素在大鼠引起镇痛效应的性别差异  

Gender Difference of the Antinociceptive Effect Induced by Intrathecal Nicotinic Acetylcholine Receptor Agonist Epibatidine in Rats

作　　者：崔宇[1] 夏杰华[2] 张旭宇[2] 胡芬[1] 陈宇[2] 

机构地区：[1]中山大学中山医学院生理教研室,广东广州510080 [2]中山大学附属第一医院麻醉科,广东广州510080

出　　处：《中山大学学报（医学科学版）》2011年第5期582-587,共6页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划(2009B030801110);广东省卫生厅医学科研基金(B2009064)

摘　　要：【目的】探讨烟碱型乙酰胆碱受体(nAChR)激动剂蛙皮素引起镇痛效应的性别差异及其可能的受体机制。【方法】采用痛行为学测试观察鞘内注射蛙皮素在雌雄大鼠引起的镇痛效应以及其拮抗剂美加明对抗其镇痛作用的差异性;利用免疫荧光组织化学方法,观察雌雄大鼠脊髓背角nAChR亚单位α4的表达是否存在性别差异,以探讨蛙皮素镇痛效应性别差异的可能机制。【结果】①鞘内给予蛙皮素剂量依赖性地发挥镇痛作用,且在雌性大鼠的镇痛作用大于雄性大鼠;②nAChR特异的非竞争性拮抗剂-美加明能够阻断蛙皮素的镇痛作用,且阻断效应于雄鼠大于雌鼠;③介导蛙皮素镇痛作用的nAChRα4表达于脊髓背角神经元,但雌雄大鼠间的表达未发现性别差异。【结论】鞘内注射蛙皮素产生的镇痛效应在大鼠具有性别差异,但蛙皮素受体nAChRα4的表达可能与镇痛效应的差异性没有关系。【Objective】 To investigate the gender difference of the antinociceptive effect induced by intrathecal nicotinic acetylcholine receptor agonist epibatidine in rats and the potential receptor mechanisms underlying such gender difference.【Method】 The antinociceptive behavioral test was performed to observe the difference of analgesia induced by intrathecal epibatidine,as well as the inhibition of mecamylamine on the analgesia,between male and female rats.The nAChRα4 expression was observed by immunofluorescence staining to investigate the mechanism underlying the gender difference of epibatidine-induced analgesia.【Result】 Intrathecal injection of epibatidine induced analgesia in dose-dependent manner with more potent analgesic effect in female rat than male rat.Although non-competent antagonist of nAChR mecamylamine pretreatment partially blocked the analgesia induced by epibatidine,stronger analgesic effect maintained in female than male rats.Immunohistochemistry staining showed that nAChRα4,which mediated the analgesia of epibatidine,was expressed in the neurons of the lumber spinal dorsal horn.However,no difference in the nAChRα4 expression was detected between male and females rats.【Conclusion】 There is gender difference in the antinociceptive effect induced by intrathecal epibatidine between male and female rats.Mechanisms other than the expression of nAChRα4 might underlie such gender difference.

关 键 词：镇痛 蛙皮素 烟碱型乙酰胆碱受体 脊髓 

分 类 号：R6[医药卫生—外科学]