Expression of nerve growth factor precursor, mature nerve growth factor and their receptors during cerebral ischemia-reperfusion injury  

作　　者：Guoqian He Jian Guo Jiachuan Duan Wenming Xu Ning Chen Hongxia Li Li He 

机构地区：[1]Department of Neurology, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China [2]National Chengdu Center for Safety Evaluation of Traditional Chinese Medicine, West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China [3]Joint Laboratory of Reproductive Medicine, Sichuan University-The Chinese University of Hongkong Joint Laboratory for Reproductive Medicine （SCU-CUHK）, Institute of Women and Children＇s Health, Department of Obstetrics and Gynecology, West China Second University Hospital, Sichuan University, Chengdu 610041, Sichuan Province, China

出　　处：《Neural Regeneration Research》2011年第22期1701-1708,共8页中国神经再生研究（英文版）

基　　金：the National High Technology Program of China (863 Programs), No. 2006AA02A117

摘　　要：We investigated nerve growth factor precursor （proNGF） and mature NGF expression in ischemic and non-ischemic cortices after cerebral ischemia-reperfusion injury. In both ischemic and non-ischemic cortices, proNGF was found to be present in the extracellular space and cytoplasm. In addition, mature NGF was expressed in extracellular space, but with a very low signal. In ischemic cortex only, proNGF was significantly decreased, reaching a minimal level at 1 day. Mature NGF was increased at 4 hours, then reached a minimal level at 3 days. The p75 neurotrophin receptor （p75NTR） was significantly decreased after ischemia, and increased at 3 days after ischemia. These results confirmed that proNGF was the predominant form of NGF during the pathological process of cerebral ischemia-repeffusion injury. In addition, our findings suggest that ischemic injury may influence the conversion of proNGF to mature NGF, and that proNGF/p75NTR may be involved in reperfusion injury.We investigated nerve growth factor precursor （proNGF） and mature NGF expression in ischemic and non-ischemic cortices after cerebral ischemia-reperfusion injury. In both ischemic and non-ischemic cortices, proNGF was found to be present in the extracellular space and cytoplasm. In addition, mature NGF was expressed in extracellular space, but with a very low signal. In ischemic cortex only, proNGF was significantly decreased, reaching a minimal level at 1 day. Mature NGF was increased at 4 hours, then reached a minimal level at 3 days. The p75 neurotrophin receptor （p75NTR） was significantly decreased after ischemia, and increased at 3 days after ischemia. These results confirmed that proNGF was the predominant form of NGF during the pathological process of cerebral ischemia-repeffusion injury. In addition, our findings suggest that ischemic injury may influence the conversion of proNGF to mature NGF, and that proNGF/p75NTR may be involved in reperfusion injury.

关 键 词：cerebral ischemia-reperfusion injury nerve growth factor precursor mature nerve growth factor p75 neurotrophin receptor cell apoptosis 

分 类 号：Q95-33[生物学—动物学] Q593.2