西藏墨脱县多斑按蚊复合体尚未发生击倒抗性突变  

No knockdown resistance was found in Anopheles maculatus complex in Motuo County of Tibet

作　　者：刘茜[1] 武松[1] 汤林华[2] 周水森[2] 黄芳[2] 叶苗青[1] 洪结凤[1] 俞俊峰[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,合肥230038 [2]中国疾病控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,上海200025

出　　处：《中国人兽共患病学报》2011年第9期801-803,共3页Chinese Journal of Zoonoses

基　　金：安徽省自然科学基金(No.10040606Q38);卫生行业科研专项(No.200802021);第五轮中国全球基金疟疾项目实施性研究专项基金(No.GF/M5/2010-04);安徽省优秀青年教师基金项目(No.2009SQRZ119)

摘　　要：目的探明西藏林芝地区疟疾流行区墨脱县多斑按蚊复合体是否发生击倒抗性突变,并获得我国多斑按蚊复合体5成员种钠离通道蛋白基因(VGSC)IIS4-S6区段基因序列信息。方法采用简并引物扩增我国多斑按蚊复合体5成员种的VGSC基因IIS4-S6区段,扩增产物双向测序拼接;PCR扩增墨脱县背崩乡、德兴乡、墨脱镇和达木乡捕获的共161只多斑按蚊复合体VGSC基因,对PCR产物双向测序,观察L1014位点是否发生突变。结果获得我国多斑按蚊复合体5成员种VGSC基因IIS4-S6区段基因序列,5成员种L1014位点均为TTA,对墨脱县的背崩乡、墨脱镇和达木乡捕获的伪威氏按蚊和威氏按蚊进行VGSC扩增并测序,均未发生击倒抗性突变。结论西藏林芝地区墨脱县多斑按蚊复合体尚未发生击倒抗性突变。The purpose of this study was to testify the knockdown resistance mutation of Anopheles maculatus complex in Motuo County and obtain the voltage-gated sodium channel IIS4-S6 segment nucleotide sequence of five members of Anopheles maculatus complex in China.Degenerate primers were used to amplify the five members of Anopheles maculatus complex,and the PCR results were sequenced and blasted with bioedit software.The result indicated that no L1014 Kdr mutation were found in group of 161 Anophles maculatus selected for the detection.

关 键 词：多斑按蚊复合体 钠离子通道蛋白 击倒抗性 西藏 墨脱 

分 类 号：R382.3[医药卫生—医学寄生虫学]