表皮干细胞分化结膜上皮细胞基因检测的实验研究  

Studies on Specifity Detection of Transdifferentiation of Epidermal Stem Cell to Conjunctival Epithelium

作　　者：孟繁剑[1] 陈家祺[2] 

机构地区：[1]广州市红十字会医院眼科,广东广州510220 [2]中山大学中山眼科中心,广东广州510060

出　　处：《中国现代医生》2011年第27期10-12,共3页China Modern Doctor

基　　金：国家自然科学基金资助项目(30271388);卫生部临床学科重点项目基金赞助(2004468)

摘　　要：目的通过实时RT-PCR方法检测经定向诱导分化的表皮干细胞分子生物学特性,探讨干细胞疗法用于眼表重建的可行性。方法实验组细胞以结膜上皮细胞为饲养细胞进行共培养,并设立空白对照组细胞培养相同时间,分别于第1、3、5、7天取各组细胞提取RNA进行实时RT-PCR定量检测β2-微球蛋白、角蛋白K13基因的表达水平。结果实验组细胞共同培养3d后出现β2-微球蛋白、角蛋白K13基因的表达,第5、7天的表达量显著提高(P<0.01)。结论经本实验方法定向诱导分化后表皮干细胞可表达与正常结膜上皮细胞相类似的分子生物学特征。Objective To detect the transdifferentiation epidermal stem cells using realtime RT-PCR, and to ensure the feasibility of stem cell treatment on ocular reconstruction. Methods The experimental group cells were co-cultured with the conjunctival epithelial cells as the feeder cells with setting the blank control group. The both groups were cultured for the same time, and after 1, 3, 5, 7 day（ s ） extracted the RNA and detected the expression of genes beta-2-microglobulin and keratin 13 by real-time RT-PCR method, Results Since the third day there were expression of genes beta-2-microglobulin and keratin 13 in the experimental group, and on the fifth and seventh day the expression increased significantly （P 〈 0.01 ）. Conclusion The transdifferentiation epidermal stem cells in this study possessed the same biological characteristics as the normal conjunctival epithelium.

关 键 词：皮肤干细胞 结膜上皮细胞 实时RT—PCR 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]