微量失血对左肝外叶切除+定容失血性休克大鼠生存率的影响  

Effect of Small Amount of Blood Loss on Survival Rate of Rats with Left Hepatic Lobectomy and Fixed Volume Hemorrhagic Shock

作　　者：俞晓军[1] 钱琤[1] 张育瑆[1] 胡志前[1] 

机构地区：[1]上海第二军医大学附属长征医院普外科,上海200003

出　　处：《现代生物医学进展》2011年第19期3615-3617,共3页Progress in Modern Biomedicine

摘　　要：目的:观察在大鼠左肝外叶切除+定容失血性休克模型制作过程中微量失血对最终大鼠生存率的影响。方法:45只SD大鼠按不同的放血比例分为三组,以Wigger改良法建立左肝外叶切除+定容失血性休克模型,严格标化放血前的各项手术操作和观测指标,比较三组大鼠最终生存率的差异。结果:在分别按2.4ml/100g(A组)、2.5ml/100g(B组)、2.6ml/100g(C组)比例定容失血的大鼠模型中,各组大鼠生存率分别为:A组66.67%,B组42.86%,C组7.69%,A、B两组与C组大鼠的生存率相比较存在明显差异(P<0.05)。结论:即使是0.1ml/100g的微量失血对于该模型大鼠的生存率也是有显著影响的,这一点在大鼠的定容失血性休克模型实验中是不应被忽视的。Objective： To observe the effect of trace blood loss to the survival of rats suffered left lobular hepatectomy and fixed volume hemorrhagic shock. Methods： Forty-five Sprague-Dawley rats were separated into 3 groups according to the different proportion of bleeding. Left lobular hepateetomy and fixed volume hemorrhagic shock rats model was made referring to Wigger＇s with strict standard of all the surgical procedure and observations. The final survival rates of rats were analyzed among the three groups. Results： Among the three groups of left lobular hepatectomy and fixed volume hemorrhage rats, which classified by the different proportions of bleeding as 2.4ml/100g （group A）, 2.5ml/100g （group B）and 2.6ml/100g （group C）, survival rate of rats in each group were A of 66.667%, B of 42.86% and C of 7.69%, and there was significant difference of the survival rate between the group A/B and the group C. Conclusion： Even 0.1ml/100g trace blood loss also may lead to a conspicuous impact to the survival of the rats. This should not be ignored in some fixed volume hemorrhagic shock model of rats.

关 键 词：大鼠休克模型 失血性休克 微量失血 

分 类 号：Q95-3[生物学—动物学] R641[医药卫生—外科学]